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¡¶±±·½Ô°ÒÕ¡·[ISSN:1001-0009/CN:23-1247/S]

Issue:

2017Äê15

Page:

18-24

Research Field:

Publishing date:

Info

Title:

Identification and Characterization of ª«CBLª« Family Genes in Watermelon 

Author(s):

ZHU Qianglong; ZHAO Yulong; LYU Huiling; LUAN Feishi; GAO Peng

(College of Horticulture and Landscape Architecture£¬Northeast Agricultural University/Key Laboratory of Biology and Genetic Improvement of Horticulture Crops (Northeast region)£¬Ministry of Agriculture£¬Harbin£¬Heilongjiang 150030)ª¤

Keywords:

watermelon(ª«Citrullus lanatusª«); ª«CBLª« gene; sequence characterization; protein structure; phylogeny; ª«cisª«-elements

PACS:

-

DOI:

10.11937/bfyy.20170580

Abstract:

Plant ª«CBL ª«family genes play important roles in response to environmental stimuli.However,the available knowledge of ª«CBLª« in watermelon is insufficient.In this study,seven ª«CBL ª«genes (ª«ClaCBL1-ClaCBL7ª«) ¡¼STBZ¡½were annotated in watermelon genome by bioinformatics method and their genomic distribution,molecular feature,phylogeny and ª«cisª«-elements were also analyzed for providing valuable information for further functional dissection of watermelon ª«CBL ª«genes.The results showed that watermelon ª«CBL ª«genes were unevenly distributed in genome,and only one (ª«ClaCBL4¡¼STBZ¡½ª«) had nine exons and the rest of them had eight exons.The putative coding sequence length of ª«CBLª« ranges from 639 bp to 738 bp.On the basis of phylogeny,they were divided into three groups.Their coded proteins had FPSF motif£¬which interacted with CIPK kinases£¬and three EF-hand motifs£¬which could bind calcium ions.The putative proteins of ClaCBL were all localized into different subcellular locations except ClaCBL1 in extracellular matrix.In addition,there are several ª«cisª«-elements existed in upstream sequences of watermelon ª«CBLª« genes,which could response to different environmental stimuli and phytohormones.And the ª«cisª«-element types and number of different watermelon ª«CBLª« genes were not identical.These results indicated that watermelon ª«CBLª« genes might involve in multiple biological processes and they had different functions.

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Last Update: 2017-08-03