|Table of Contents|

Medium Optimization for Cordycepin Production From  Cordyceps militaris by Submerged Fermentation(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年05
Page:
134-141
Research Field:
Publishing date:

Info

Title:
Medium Optimization for Cordycepin Production From  Cordyceps militaris by Submerged Fermentation
Author(s):
ZHANG Nan; LI Yong; XU Jie; XIONG Mao; ZHOU Xin; DUAN Huiguo
(Key Laboratory of Regional Characteristic Agricultural Resources，Department of Education，Neijiang，Sichuan 641100)
Keywords:
Cordyceps militari; submerged fermentation; cordycepin; mycelial growth; medium; optimization
PACS:
-
DOI:
10.11937/bfyy.201705032
Abstract:
In order to explore the optimal preparation method of liquid seed and medium for cordycepin production in submerged cultivation of Cordyceps militaris (L.) Link NS-810，effect of liquid seed on mycelial growth by inoculating different concentrations of spores in seed culture was investigated.Single factor experiment and orthogonal experiment were used to select the optimal medium compositions for cordycepin production in liquid fermentation.The results showed that the best concentration of spores for first-degree spawn was 3.0×108 cfu?mL-1.Under this condition，the pale yellow hypha ball dispersed homogeneously in the seed liquid and their diameters were in the range of 1.1 mm to 1.3 mm，and the seed liquid was clear and transparent.Furthermore，the optimal media was composed of the following factors:glucose 25 g?L-1，potato 100 g?L-1，peptone (fish) 18 g?L-1，(NH4)2SO4 0.8 g?L-1，KH2PO4 1.0 g?L-1，MgSO4?7H2O 0.5 g?L-1，silkworm pupa powder 5.0 g?L-1，water 1 L.This optimization strategy enhanced the production of cordycepin nearly 1.46 times compared with the initial level.Low-cost glucose and peptone (fish) were used as quick-release carbon source and organic nitrogen source of fermentation medium respectively，which was beneficial for industrial production of cordycepin from the strain NS-810 by submerged fermentation.

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Last Update: 2017-03-24