|Table of Contents|

Establishment and Optimization of ISSR-PCR System of Pleurotus ostreatus by PAGE Detection Technology(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2017年02
Page:
91-95
Research Field:
Publishing date:

Info

Title:
Establishment and Optimization of ISSR-PCR System of Pleurotus ostreatus by PAGE Detection Technology
Author(s):
ZHANG YunfengQU AnjiangFU Xinmeng
(Department of Life Sciences,Tangshan Normal University,Tangshan,Hebei 063000)
Keywords:
Pleurotus ostreatusISSR-PCRPAGE
PACS:
-
DOI:
10.11937/bfyy.201702022
Abstract:
The separation efficiency of ISSR-PCR product was detected by agarose gel electrophoresis and different concentrations of polyacrylamide gel (PAGE) electrophoresis,with Pleurotus ostreatus‘Tangping26’genomic DNA as template and with primers U836 as primer.The results showed that the separation of PAGE was significantly better compared to agarose gel.Main factors which had an influence on ISSR reaction system were optimized based on the PAGE detection technology,that the optimum ISSR-PCR reaction system was 20 μL total volume including 20 ng template,1.5 mmol?L-1 Mg2+,0.8 μmol?L-1 primer,200 μmol?L-1 dNTPs,1.0 U Taq enzyme,30 cycles of amplification.

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Last Update: 2017-03-22