The five-year-old ginseng soil in Songjiang town Fusong county of Jilin Province was conducted as the experimental objects,respectively adding different fertilization of organic fertilizer,urea,potash fertilizer,compound fertilizer and quantitative Yishengyuan,Yiwei,DND agent,organic nitrogen and potassium fertilizer,and the fertilizer was not applied for control,effects of fertilization treatment on ginseng soil protease activity were determined.The results showed that the optimal performance of the soil protease was improved by 50 g?m-2in June,and it was an important measure to improve the soil enzyme activity.