Optimization of Cultivation Condition in Gastrodia elata Seed-germinating Strain(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Optimization of Cultivation Condition in Gastrodia elata Seed-germinating Strain

Author(s):: CHENG Lijun1; LI Shihui1; HU Zhifang2; DUAN Xiangcheng1; ZHAO Yanjuan1; (1.College of Chemistry and Life Sciences，Zhaotong University/Zhaotong Characteristic Agricultural Products Research Center，Zhaotong，Yunnan 657000；2.Zhaotong Apple Industry Research Institute，Zhaotong，Yunnan 657000)

Keywords:: Gastrodia elata; germinating strain; environmental condition; nutrient condition

Abstract:: Gastrodia elata seed-germinating strain MY-001 was used as test material，the cultivation conditions of MY-001 was studied according to the index of mycelial growth rate and mycelial biomass by single factor experiment and orthogonal experiment.The results showed that the optimum growth environmental conditions were 25 ℃，200% water content，under dark cultivation;glucose and yeast extracts were the optional carbon source and nitrogen sources.The optimum growth nutrient conditions were determined yeast extracts 3 g?L-1，glucose 20 g?L-1，composite vitamin B 0.005 g?L-1.Through multiple regression analysis to establish regression equation (Y=0.362+0.084A-0.014B-0.007C)，variance analysis showed significant equation was very significant.

[1]李德勋,张观敏,马跃新.昭通天麻的原植物调查与鉴定[J].现代中药研究与实践,2005,19(1):29-31.

[2]徐锦堂.中国天麻栽培学[M].北京:北京医科大学中国协和医科大学联合出版社,1993.

[3]程立君,陈玉惠,徐畅,等.昭通天麻种子萌发菌的筛选[J].安徽农业科学,2009,37(28):13600-13602.

[4]徐锦堂,郭顺星,范黎,等.天麻种子与小菇属真菌共生萌发的研究[J].菌物系统,2001,20(1):137-141.

[5]郭顺星,王秋颖.促进天麻种子萌发的石斛小菇优良菌株特性及作用[J].菌物系统,2001,20(3):408-412.

[6]卯晓岚.中国大型真菌[M].郑州:河南科技出版社,2000.

[7]彭述敏,陈玉惠,程立君,等.2株优良天麻共生蜜环菌生长条件筛选[J].中国食用菌,2010,29(4):22-25.

[8]徐锦堂,郭顺星.供给天麻养种子萌发营养的真菌紫萁小菇[J].真菌学报,1989,8(3)：221-226.

[9]范黎,郭顺星,曹文岑,等.墨兰共生真菌一新种的分离、培养、鉴定及其生物活性[J].真菌学报,1996,15(4):251-255.

[10]范黎,郭顺星,肖培根.天麻种子萌发过程与开唇兰小菇的相互作用[J].菌物系统,2001,20(4):539-546.

[11]宁丽,郭立忠,李翠翠,等.天麻萌发菌株的生物学特性研究[J].氨基酸和生物资源,2007,29(2):5-7.

Last Update: 2016-11-09