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Induced Resistance of Cucumber Against Botrytis cinerea by Bacillus subtilis BSD-2 CHEN Yanguang,YIN Shuli,LIU Hongwei,ZHANG Genwei,CHENG Huicai,ZHANG Liping(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年12
Page:
119-123
Research Field:
Publishing date:

Info

Title:
Induced Resistance of Cucumber Against Botrytis cinerea by Bacillus subtilis BSD-2 CHEN Yanguang,YIN Shuli,LIU Hongwei,ZHANG Genwei,CHENG Huicai,ZHANG Liping
Author(s):
(Institute of Biology; Hebei Academy of Sciences; Shijiazhuang; Hebei 050081)
(Institute of Biology,Hebei Academy of Sciences,Shijiazhuang,Hebei 050081)
Keywords:
Bacillus subtilis BSD-2; grey mould; defense enzyme
PACS:
-
DOI:
10.11937/bfyy.201612029
Abstract:
Taking Bacillus subtilis BSD-2 as test bacteria,the cucumber variety ‘ Jinchun No.4’ as test material,the effects of fermented liquid,antibacterial peptides,spore dilute solution of BSD-2 on the activities of POD,PPO,PAL,SOD in cucumber leaves were determinated by using physiological and biochemical methods.The changes of POD isozyme and leaf intercellular space proteins were also analyzed by SDS-PAGE.The result showed that the activities of POD,PPO,PAL and SOD in cucumber leaves increased significantly after the treatments of fermented liquid,antibacterial peptide and spore dilute solution,then reached the peak on the fifth day.POD isozyme expression increased both in amount and kinds.SDS-PAGE analysis suggested that a new 27 kDa protein was expressed.All aboves showed that B.subtilis BSD-2 and its antibacterial substance could improve the defense enzymes activities,and promote the expression of the POD isozyme and intercellular space related proteins,and induce resistance of cucumber to grey mould.

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Last Update: 2016-07-21