|Table of Contents|

Effect of Different Preservation Methods on Genomic DNA Extraction of Pomegranate(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年08
Page:
100-103
Research Field:
Publishing date:

Info

Title:
Effect of Different Preservation Methods on Genomic DNA Extraction of Pomegranate
Author(s):
CHEN Yun1; 2; GAO Yuanqing1; WANG Jilian1; 2; MA Liufeng1; 2
(1.College of Biology and Geography Science，Kashgar University，Kashgar，Xinjiang 844006;2.The Key Laboratory of Ecology and Biological Resource in Yarkant Oasis at Colleges and Universities Under the Department of Education of Xinjiang Uygur Autonomous Region，Kashgar，Xinjiang 844006)
Keywords:
Punica granatum L.; preserved methods; DNA extraction
PACS:
-
DOI:
10.11937/bfyy.201608028
Abstract:
Taking leaves of Punica granatum L.as materials，six methods of preserving fresh leaves were designed，genomic DNA was extracted by improved CTAB method，the effect of different preserving methods on genomic DNA extraction was studied.The results showed that the DNA extracted from leaves stored about 6 months at the -70℃ and 5 days at 4℃ could get the same high quality comparing with the fresh leaves.Genomic DNA also could be extracted from sample stored 7 and 14 days at -20℃，but the concentration was slightly lower than that extracted from fresh leaves.However，DNA from silica gel-dried sample was not obtained by the same way.After optimization of the DNA extraction method，the high-purity DNA from silica gel-dried sample was got,but the concentration of DNA were about 50% comparing with fresh leaves.Genomic DNA extracted from samples preserved as the above-mentioned six methods could meet the demands for SRAP amplification.Clear and perfect SRAP amplification patterns were obtained.

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Last Update: 2016-06-30