Tissue Culture and Virus Elimination Technique for Apple Dwarf Rootstock(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Tissue Culture and Virus Elimination Technique for Apple Dwarf Rootstock

Author(s):: YANG Yanmin; WEI Yongxiang; LIU Cheng; ZHANG Duo; WANG Xingdong; LIU Youchun; (Liaoning Institute of Pomology，Yingkou，Liaoning 115009)

Keywords:: apple; dwarf rootstock; tissue culture; rapid propagation; virus elimination

Abstract:: Taking ‘Mark 9’,‘Liaozhen No.2 ’and ‘GM256’ apple dwarf rootstock varieties which were commonly used in Liaoning as materials，the technique of tissue culture and heat treatment were studied to establish a rapid propagation system for virus-free apple dwarf rootstock.The results showed that for ‘Mark 9’，MS+6-BA 1.5 mg/L+NAA 0.1 mg/L，6-BA 0.8 mg/L+NAA 0.1 mg/L and 1/2MS+IBA 0.5 mg/L+NAA 0.3 mg/L were the best media for inducing，proliferating and rooting，respectively，induction rate was 85.7%；for ‘Liaozhen No.2’，MS+6-BA 1.5 mg/L+NAA 0.1 mg/L and 6-BA 0.5 mg/L+NAA 0.1 mg/L were the best media for inducing and proliferating，respectively，induction rate was 87.5%，the rooting medium same as ‘Mark 9’；for ‘GM256’，MS+6-BA 1.0 mg/L+NAA 0.1 mg/L and 6-BA 0.8 mg/L+NAA 0.1 mg/L were the best media for inducing and proliferating，respectively，induction rate was 78.5%，the rooting medium same as ‘Mark 9’.The heat resistance of ‘Mark 9’ was the strongest，followed by the ‘GM256’ and ‘Liaozhen No.2’.The effect of virus elimination was the best with heat treatment for 40 days，the virus-free rate were 53.33%，66.67% and 71.43% for ‘Mark 9’，‘Liaozhen No.2’ and ‘GM256’，respectively.Treated for 5 minutes by 1 000×cytex，the survival rate of transplant were the highest，of which 93.33%,92.00%,87.50% for three varieties tested，respectively.

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Last Update: 2016-04-21