|Table of Contents|

Research on Intracellular Polysaccharide Yield and Extracellular Enzyme Activity Change of Armillarilla Strains Isolated From Guizhou(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2016年02
Page:
134-138
Research Field:
Publishing date:

Info

Title:
Research on Intracellular Polysaccharide Yield and Extracellular Enzyme Activity Change of Armillarilla Strains Isolated From Guizhou
Author(s):
HUANG Wanbing1; 2; GUI Yang1; 2; GONG Guanglu1; 2; YANG Tongjing1; 2; LIU Chaogui3; ZHU Guosheng1; 2
(1.Institute of Morden Chinese Medicinal Materials of Guizhou Province，Guiyang，Guizhou 550006;2.Guizhou Key Laboratory of Agricultural Biotechnology，Guiyang，Guizhou 550006；3.School of Horticulture and Landscape Architecture，Southwest University，Chongqing 400716)
Keywords:
Armillarilla; xylanase; CMCase; laccase; intracellular polysaccharide
PACS:
-
DOI:
10.11937/bfyy.201602035
Abstract:
Taking Armillarilla strains isolated from Guizhou as test material，using phenol-sulfuric acid method to determine the content of total polysaccharide，the DNS method and syringaldazine were applied to measure the enzyme activity of xylanase，carboxymethyl cellulose enzyme and laccase，the change rule of extracellular enzyme activity and intracellular polysaccharide yield of different Armillarilla hyphae came from liquid fermentation were studied.The results showed that，there were significant differences between polysaccharide yield of different strains.The change rule of the extracellular different enzyme activity were same.They all rose at first and lowered later.The maximum enzyme activity and the time when enzyme activity reach maximum between different strains were also obvious difference.The fine strain DJ1 could end fermentation on the fourth day.Its maximum enzyme activity was always the earliest to appear and at the latest to disappear.When liquid fermentation，Armillaria prior secreted CMC enzyme and laccase，then secreted xylanase.But xylanase played a main role.

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Last Update: 2016-04-07