Study on Inhabition Methods to Miscellaneous Microorganism for Soil Actinomycetes Isolation(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Study on Inhabition Methods to Miscellaneous Microorganism for Soil Actinomycetes Isolation

Author(s):: KANG Pingzhi1; WU Xiuhong2; ZHANG Lirong1; (1.Institute of Plant Protection，Ningxia Academy of Agriculture and Forestry Sciences/Ningxia Key Laboratory of Plant Disease and Insect Pest Control,Yinchuan,Ningxia 750002;2.Ningxia Tongxin County Forestry Bureau,Tongxin,Ningxia 751300)

Keywords:: soil actionmycetes; isolation; miscellaneous microorganism; inhabition methods

Abstract:: The influence of different pretreatment ways of the soil samples and miscellaneous microorganism inhibitors to the isolation effect of soil actinomycetes was studied by plate paint isolation method.The results showed that actinomycetes isolation effect of air drying (7-10 days) soil samples was better than that of heat treatment (120℃×1 hour).The number of actinomycetes isolated and kinds were more,and easily purified,and miscellaneous microorganism (bacteria，fungi) also decreased significantly.However，actinomycetes were hardly isolated in the soil samples that were treated with 120℃×1 hour，bacteria number was still more,fungi were all killed;by testing repeatedly four kinds of miscellaneous microorganism inhibitors and their combinations,suitable inhibitor(S+Q) and its optimal dosage of soil actinomycetes isolation and purification were selected out.The optimum dosages of potassium sorbate(S) and ampicillin sodium(Q) were 30 mg/L and 3 mg/L,respectively.Potassium dichromate (Z) and its combinations(Z+Q) also had certain inhibitory effect on soil bacteria and fungi,but their inhibitory effect were not as well as those of S+Q，and isolation number of actinomycetes also were lower.Therefore,through double dealing with air drying soil samples and adding S+Q in actinomycetes isolation medium(GA),better effect of soil actinomycetes isolation and purification were available.

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Last Update: 2016-02-17