Study on Tissue Culture and Rapid Propagation of Gynura formosana Kitam(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Study on Tissue Culture and Rapid Propagation of Gynura formosana Kitam

Author(s):: MU Hongmei1; DU Xiuju2; CAO Xing1; WU Yan1; SUN Kai1; （1.College of Agriculture，Liaocheng University，Liaocheng，Shandong 252059；2.College of Life Science，Liaocheng University，Liaocheng，Shandong 252059）

Abstract:: The stem of Gynura formosana Kitam was used as explants，tissue culture method was used，and the effect of mercuric chloride soaking time and different hormone combinations on the induction of stem segment of Gynura formosana Kitam were studied.The results showed that，when the explants’ sterilization time was 11 minutes with 0.1% mercuric chloride solution，the contamination rate of explants was minimum (41%)，the survival rate was 68%.The stem suitable induction culture medium was MS+6-BA 3.0 mg/L+NAA 0.02 mg/L，at this time the induction rate reached 98%，the number of buds per explants reached 8.0.The suitable induction culture of rooting medium was 1/2 MS+NAA 0.20 mg/L.

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Last Update: 2015-12-23