Diversity of Endophytic Fungi in Roots of Blueberry(PDF)
《北方园艺》[ISSN:1001-0009/CN:23-1247/S]
- Issue:
- 2015年21
- Page:
- 25-30
- Research Field:
- Publishing date:
Info
- Title:
- Diversity of Endophytic Fungi in Roots of Blueberry
- Author(s):
- LIU Fenghong
- (Agricultural College，Ludong University，Yantai，Shandong 264025)
- Keywords:
- :isolation frequency; dominant population; diversity index; similarity coefficient
- PACS:
- -
- DOI:
- 10.11937/bfyy.201521007
- Abstract:
- Shandong is one of the major blueberry growing areas in China.By the end of 2014，the planting area was about 3 000 hm2 and the annual production was about 50 000 tons.In order to explore the community structure and distribution pattern of endophytic fungi in roots of blueberry，healthy root samples were collected from four main planting locations，which were Qingdao，Tai’an，Weihai and Yantai.The sampled blueberry belonged to 2 cultivars，viz.，‘Bluecrop’ and ‘Duke’.A total of 1 345 strains of endophytic fungi were isolated from root samples.Based on morphological methods and molecular techniques，29 genera were identified，including one genus of Oomycetes (0.45%)，two genera of Basidiomycetes(2.15%)，seventeen genera of Ascomycetes(31.16%)，nine genera of Fungi Imperfecti(60.82%)，the residual 5.42% of all isolates were not identified.The results showed that plentiful fungal diversity was present in the roots of cultivated blueberry plant in Shandong Province, of which the species in Fusarium was the predominant groups of endophtic fungi，its relative isolation frequency was 31.67%.The species of Trichoderma，Penicillium，Cladosporium and Zalerion were the subdominant groups.The fungal population structures and composition of endophytic fungi varied with cultivars and sites，and also had regularity to a certain extent.Fusarium occurred in all the 2 investigated blueberry plants.The fungal diversities in roots of the two blueberry plants all grown in four investigated sites were significantly different.‘Duke’ of Weihai was the highest in Shannon diversity index(H′=2.835 9) and Margalef richness index (R=4.264 3).‘Duke’ of Tai’an was the highest in Pielou evenness index(E=0.939 1).The similarity coefficients of fungal populations in roots of blueberry were highest in Weihai and Qingdao(Cj=0.57 and Cs=0.73).
Last Update: 2015-12-22