Spore Propagation Techniques of Osmunda cinnamomea L.var.asiatica(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Author(s):: XIE Xiuhong1; LI Wei2; LI Cuilan3; LIANG Yunjiang2; (1.College of Landscape Architecture,Changchun University,Changchun,Jilin 130022；2.College of Agriculture,Yanbian University，Yanji，Jilin 133002;3.College of Resource and Environmental Science,Jilin Agricultural University,Changchun,Jilin 130118)

Abstract:: Culture conditions of spore germination，gametophyte growth and sporophyte transformation of Osmunda cinnamomea L.var.asiatica were preliminary discussed in this paper with different conditions(substrates,temperature,light) and different concentration of KH2PO4,hormone,water,ABA，to provide theoretical foundation of artificial rapid propagation techniques for making use of spores.The results showed that favorable conditions of spore germination and gametophyte development were as follows：a part of protective ground soil and a part of peat soil were mixed as seeding substrate，spores were sowed after 100℃ high temperature sterilization with maintaining conditions of natural temperature and alternating day and night lighting.3 days after sowing spore，spraying 2,4-D once every 3 days，continuous sprayed 3 times.Spraying 0.3% KH2PO4 once every 3 days，continuous sprayed 5 times.Favorable conditions of sporophyte transformation and development were as follows：when gametophyte was saw by naked eye，transplanting good gametophyte，adjusting water content of substrate to field capacity.A week after transplanting，spraying 1.0 mg/L hormone ABA once every 3 days，continuous sprayed 3 times.

[1]马玉心,高德武,蔡体久.分株紫萁的研究进展[J].国土与自然资源研究,2001(1):95-96.

[2]李武.薇菜及其利用现状[J].中国野生植物,1989(3):22-23.

[3]周永杰,张万华.薇菜人工栽培与加工技术[M].北京：中国农业出版社，2004.

[4]梁运江,李伟,王维娜,等.薇菜的生物学特性及人工繁殖[J].北华大学学报(自然科学版),2010,11(2):183-185.

[5]何义发,罗世家,王柏泉,等.薇菜孢子繁殖育苗试验初级[J].中国林副特产,1995(4):8-9.

[6]高德峰,杨万森,周立刚.野生薇菜的人工高产栽培技术[J].湖北农业科学,2001(5):68-69.

[7]何义发.经济蕨类植物紫萁的研究进展与展望[J].湖北农业科学,2002(6):101-103.

[8]王谋强,张朝君.我国南方薇菜的研究现状及其发展对策[J].贵州农业科学,2006,34(4):135-136.

[9]鲍士旦.土壤农化分析[M].3版.北京:中国农业出版社,2000.

[10]依艳丽.土壤物理研究法[M].北京:北京大学出版社,2009.

[11]HASGAWA N.On the sexual generation of three species of Osmunda in Japan[J].Journ Jap Bot，1955，30(3):97-101.

[12]包文美,敖志文,刘保东.东北蕨类植物配子体发育的研究-Ⅲ.紫萁科[J].植物研究,1986,6(3):117-125.

Last Update: 2015-11-17