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Preliminary Study on Adventitious Bud Induction and Plant Regeneration of Cassia angustifolia(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年16
Page:
98-101
Research Field:
Publishing date:

Info

Title:
Preliminary Study on Adventitious Bud Induction and Plant Regeneration of Cassia angustifolia
Author(s):
HUANG Hao; WEI Ying; MENG Aidong
（Guangxi Botanical Garden of Medicine Plant，Nanning，Guangxi 530023）
Keywords:
medicinal plants; Cassia angustifolia; tissue culture; plant regeneration
PACS:
-
DOI:
10.11937/bfyy.201516023
Abstract:
Adventitious bud differentiation，bud seedling multiplication，root induction from mature brunch of Cassia angustifolia，and transplanting substrate of tissue culture-raised plantlets transplantation were studied by tissue culture method.The results showed that the efficient adventitious bud differentiation was cultured on MS media supplemented with 2.0 mg/L TDZ+0.1 mg/L NAA.The highest number of adventitious buds (12.6±0.64) per explant were observed in MS media supplemented with 2.0 mg/L 6-BA+0.05 mg/L NAA.The highest rooting percentage (58.5%) and survival were achieved using 1/2MS media with 2.0 mg/L IBA and 1% (w/v) active carbon.The 73.7% plantlets survived acclimatization in the greenhouse，and producing healthy plants on the field.The current investigation showed efficient in vitro regeneration capabilities of Cassia angustifolia from brunch explants.

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Last Update: 2015-09-02