Study on Optimizing Extraction Techniques ofPotentilla chinensis Ser.Polysaccharides by Response Surface Methodology(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Study on Optimizing Extraction Techniques ofPotentilla chinensis Ser.Polysaccharides by Response Surface Methodology

Author(s):: LI Min1; 2; LUO Yiyuan3; YUE Qibo1; (1.College of Traditional Chinese Medicine，Jilin Agricultural Science and Technology University，Jilin，Jilin 132101；2.Jilin Key Laboratory for Utilization and Protection of Animal and Plant Resources in Changbai Mountain,Jilin，Jilin 132101；3.College of Pharmacy，Nanjing University of Chinese Medicine，Nanjing,Jiangsu 210023)

Keywords:: Potentilla chinensis Ser.; polysaccharide; response surface method; extraction techniques

Abstract:: Taking Potentilla chinensis Ser.as test material，with the index for the content of polysaccharides in Potentilla chinensis Ser.,the effect of four factors such as solvent-solid ratio，ultrasonic power，ultrasonic temperature and extraction time were designed by Box-Behnken central composite.Extraction technic parameters of polysaccharides in Potentilla chinensis Ser.was optimized by response surface methodology.The results showed that the optimizing extraction conditions of polysaccharides in Potentilla chinensis Ser.were as follows：liquid-solid ratio was 50∶1 mL/g，ultrasonic power was 500 W，ultrasonic temperature was 63℃ and extraction time was 30 minutes.Under the conditions the extraction rate of polysaccharides was 2.448 6%.Optimizing extraction technics by response surface methodology was reasonable，simple，and had good feasibility.

[1]孙天航,刘玫,孙雪芹,等.东北委陵菜属植物叶形态结构的研究及其分类学价值的探讨[J].草叶科学,2014,23(3)：75-84.

[2]刘素君,成英,陈刚.鹅绒委陵菜多糖提取及抗氧化活性的研究[J].时珍国医国药,2011,22(9)：2103-2105.

[3]成英,宋九华,刘素君.鹅绒委陵菜多糖对荷瘤小鼠细胞因子的影响[J].安徽农业科学,2012,40(9)：5177-5178.

[4]刘素君,李世元,宋九华,等.鹅绒委陵菜多糖抗肿瘤作用研究[J].中国现代应用药学,2011,28(3)：185-188.

Last Update: 2015-08-14