Establishment of Regeneration System of Atropa belladonna(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Author(s):: ZOU Lijuan; WU Qinggui; XIE Mushan; LIU Danli; LUO Minghua; (Ecological Security and Protection Key Laboratory of Sichuan Province，College of Life Science and Technology，Mianyang Normal University，Mianyang，Sichuan 621000)

Abstract:: Taking seedlings of Atropa belladonna as explants，by tissue culture methods，MS as basic culture medium with different concentrations of IAA，NAA，6-BA and KT were used to establish the optimal media for callus induction and differentiation.The results showed that 100% of calli induction rate was obtained on MS medium with KT 0.5 mg/L+6-BA 1.0 mg/L and KT 1.0 mg/L+6-BA 0.5 mg/L.Calli could not be induced on MS medium with the combination of auxin and KT or 6-BA.The induced calli on MS media with KT or KT with 6-BA were subcultured optimally at 20 days and 40 days.1/2MS+NAA 0.2-0.4 mg/L was the optimal medium for plants’ rooting.This study would provide the technologicalsystem of tissue culture for Atropa belladonna regeneration.

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Last Update: 2015-08-14