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Genetic Diversity Analysis of Wild Germplasm Resource of Pyrus Based on SRAP Markers(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年13
Page:
1-5
Research Field:
Publishing date:

Info

Title:
Genetic Diversity Analysis of Wild Germplasm Resource of Pyrus Based on SRAP Markers
Author(s):
LIANG Ting-tingMA YanZANG De-kui
Forestry College,Shandong Agricultural University,Tai’an,Shandong 271018
Keywords:
Pyrusgenetic diversityPyrus hopeiensisPyrus trilocularisSRAP
PACS:
S 661.2
DOI:
10.11937/bfyy.201513001
Abstract:
Taking six wild species of Pyrus from different regions as materials and one P.communis as control,genetic diversity of partial germplasm resource from wild species of Pyrus was studied by sequence related amplified polymorphism (SRAP).The results showed that,199 loci were identified with 14 SRAP primer combinations out of which 194 were polymorphic. The proportion of polymorphic loci (PPL) was 97.29%,Nei’s gene diversity(H)was 0.296 1,Shannon’s Information index(I)was 0.451 9,which showed the abundant genetic diversity in Pyrus. Coefficient of genetic differentiation (Gst) was 0.646 2,Nei’s genetic identity and genetic distance were 0.512 6-0.920 1 and 0.083 2-0.668 3.The genetic distance between each pair of P.hopeihensis,P.ussuriensis,and P.phaeocarpa was obviously smaller than any other pairs,showing the closer relationship of the three species.Also,genetic relationship of P.trilocularis,P.phaeocarpa,and of P.betulaefolia,P.phaeocarpa were close,while P.calleryana was more distant with other indigenous species.The analysis result of PCOA was similar with cluster analysis,and the former directly showed the relationships between all groups.

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Last Update: 2015-08-17