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Verification of Two Excellent Auricularia auricula Species Based on Internal Transcribed Spacer(ITS) Sequences in Nyingchi Area(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年10
Page:
98-103
Research Field:
Publishing date:

Info

Title:
Verification of Two Excellent Auricularia auricula Species Based on Internal Transcribed Spacer(ITS) Sequences in Nyingchi Area
Author(s):
LIU Zhen-dong1; 2; XUE Bei1; 2; WANG Ze-min1; 2; YANG Yun-yu1; 2; ZHANG Yan-long3
(1.Department of Food Science，Tibet Agricultural and Animal Husbandry College，Nyingchi,Tibet 860000;2.Platform of Wild Characteristic Biological Resources in Tibet,Nyingchi,Tibet 860000;3.Province Key Laboratory of Microbiology，College of Life Science，Heilongjiang University，Harbin,Heilongjiang 150000)
Keywords:
Nyingchi area; Auricularia auricula; ITS; verification
PACS:
S 646.6
DOI:
10.11937/bfyy.201510024
Abstract:
Taking two excellent field Auricularia auricula species ‘X6’ and ‘X7’ in Nyingchi area as test material,ITS Sequence Analysis was used to screen,the software Clustal X 1.83 and MAGA 5.0 were used to conduct the phylogeny analysis.The results showed that two excellent field species ‘X6’ and ‘X7’ were Auricularia auricula,comparing the DNA sequence and building a common phylogenetic tree，it was confirmed that there was a certain genetic difference between the two.

References:


[1]王贺祥.食用菌栽培学[M].北京:中国农业大学出版社,2008:121-124.
[2]张金霞,谢宝贵.食用菌菌种生产与管理手册[M].北京:中国农业大学出版社,2006:5-7.
[3]李晓.黑木耳标准化生产技术[M].北京:金盾出版社,2012:21-23.
[4]张伟林.黑木耳单核杂交菌株远源性的研究[D].哈尔滨:黑龙江大学,2011.
[5]郭鑫.地栽黑木耳菌种选育、鉴定与替代原料研究[D].哈尔滨:黑龙江大学，2010.
[6]赵丽.紫外线诱变筛选黑木耳优良菌株及RAPD鉴定[D].延吉：延边大学,2009.
[7]Fernandez M，Figueiras A，Benito C.The use of ISSR and RAPD markers for detecting DNA polymorphism，genotype identification and genetic diversity among barley cultivars with known origin[J].Theoretical and Applied Genetics，2002，104(5)：845-851.
[8]莎姆布鲁克.分析克隆实验指南[M].黄培堂，译.北京:科学出版社,2008.

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Last Update: 2015-08-07