|Table of Contents|

Study on Regeneration of Thin Cell Layer of Pyrus ussuriensis(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年06
Page:
95-98
Research Field:
Publishing date:

Info

Title:
Study on Regeneration of Thin Cell Layer of Pyrus ussuriensis
Author(s):
ZHOU Jia-hong1; XING Cai-hua2 ; CAO Hui2 ; WANG De-fen2 ; YANG Chun-lei2 ; LI Ding-li2
(1.Changdao Futao Design Architectural Limited Company，Yantai,Shandong 265800;2.College of Horticulture，Qingdao Agricultural University，Qingdao，Shandong 266109)
Keywords:
Pyrus ussuriensis; thin cell layer; regeneration; stem
PACS:
-
DOI:
10.11937/bfyy.201506026
Abstract:
Regeneration system of thin cell layers (TCL)was established by using shoots of Pyrus ussuriensis as explants,to provide basis for asexual propagation and industrialized seedling production of Pyrus ussuriensis,meanwhile to provide reference for application of TCL technique in Pyrus research.The results showed that regeneration efficiency of TCL was affected by the combination and concentration of hormones.The optimal culture medium of adventitious shoots regeneration was MS+BA 1.5 mg/L+NAA 0.25 mg/L+sucrose 30 g/L+agarose 7 g/L，and the regeneration rate was 100%.The optimal culture medium of adventitious roots was 1/2MS+IBA 0.5 mg/L+sucrose 30 g/L+agarose 7 g/L，and the rate of rooting was 66.7%.Meanwhile，callus and adventitious shoots induction rate increased under dark culture.

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Last Update: 2015-06-25