|Table of Contents|

Domestication and Cultivation of Wild Pleurotus Cystidiosus and Its Molecular Identification(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年04
Page:
143-146
Research Field:
Publishing date:

Info

Title:
Domestication and Cultivation of Wild Pleurotus Cystidiosus and Its Molecular Identification
Author(s):
LI DieWU HaoLI Hong-yuLEI Yu-xiaLIU Bin
(Applied Microbiology Institute,Agricultural College,Guangxi University,Nanning,Guangxi 530003)
Keywords:
Pleurotus cystidiosusITSbiological characteristicscultivation
PACS:
-
DOI:
10.11937/bfyy.201504034
Abstract:
Taking Pleurotus cystidiosus as material,through morphology characteristics,culture characteristics,ITS sequence (Gene Bank accession number:KJ868811) and other identification to determine the strain of Pleurotus cystidiosus.To study its biological characteristics.Cultioation test was conducted by using cotton seed hull,corn cobs,saw dust,sugarcane bagasse which was abundant in Guangxi as substrate,to select formula of substrate suitable for Guangxi.The results showed that,in five kinds of the tested carbon sources,Pleurotus cystidiosus mycelium had better effect by using starch;In six kinds of selected nitrogen source,it was the best by using yeast powder;Pleurotus cystidiosus mycelia could grow in the carbon nitrogen ratio 10∶1—60∶1 range,but the 40∶1 was the best;the optimum growth temperature range were 25.0℃ and 30.0℃,with 27.5℃ was the best;the optimal pH 7.0.The cultivation experiment results showed that with the saw dust as main ingredient in its biological efficiency could reach 43.33%,the highest mushroom weight was 79.51 g/bag.

References:

[1]万鲁长,黄春燕,张柏松,等.北方大棚鲍鱼菇标准化栽培技术[J].食用菌,2005,27(6):31-32. [2]黄春燕,万鲁长,单洪涛,等.不同营养条件对鲍鱼侧耳菌丝生长的影响[J].山东科学,2006,19(6):6-11. [3]王德源,刘可春,党立,等.利用果树枝条木屑栽培鲍鱼菇技术[J].陕西农业科学,2007(3):174-175. [4]徐瑞雅,齐志广,贾耐兵,等.食用菌最佳母种培养基的筛选[J].河北农业科学,2007,11(1):23-24,45. [5]阮晓东,阮周玺,李月桂,等.鲍鱼菇代料栽培技术[J].食药用菌,2012,20(6):362-363. [6]吕作舟.食用菌栽培学[M].北京:高等教育出版社,2006:414-417. [7]孙立夫,张艳华,裴克全.一种高效提取真菌总DNA的方法[J].菌物学报,2009,28(2):299-302. [8]许峰,刘宇,王守现,等.一种适于PCR反应的快速提取食用菌基因组DNA的方法[J].生物技术,2011,21(1):43-44. [9]White T J,Bruns T,Lee S.Analysis of phylogenetic relationships by amplification and direct sequencing of ribosomal RNA genes[M]//Innins M A.PCR Protocols:A Guide to Methods and Applications.New York:Academic,1990:315-322. [10]张玉铎,徐凯,张东雷,等.九个耐高温平菇品种比较试验[J].北方园艺,2012(12):182-183. [11]侯丽冰,贺伟,刘小勇,等.我国几种松干锈菌亲缘关系的ITS序列分析[J].北京林业大学学报,2002,24(5):175-182. [12]徐学锋,李安政,程水明,等.亚洲香菇Lentinula edodes系统发育学地位的重新评估与遗传多样性分析[J].自然科学进展,2005,15(10):1204-1210.

Memo

Memo:
-
Last Update: 2015-04-21