|Table of Contents|

Cloning，Sequsence Analysis and Tissue-sepecific Expression of Methylcrotonyl-CoA Carboxylase from Cassia obtusifolia(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2015年03
Page:
99-104
Research Field:
Publishing date:

Info

Title:
Cloning，Sequsence Analysis and Tissue-sepecific Expression of Methylcrotonyl-CoA Carboxylase from Cassia obtusifolia
Author(s):
LIU Zu-bi; LI Juan-juan; ZHU Qian-kun; SONG Tao; ZHOU Jia-yu; LIAO Hai
(School of Life Science and Engineering，Southwest Jiaotong University，Chengdu,Sichuan 610031)
Keywords:
Methylcrotonyl-CoA carboxylase; Cassia obtusifolia;  gene cloning; bioinformatics; gene expression
PACS:
-
DOI:
10.11937/bfyy.201503030
Abstract:
The cDNA sequence of Cassia obtusifolia MCCase was abtained by RACE and analyzed by bioinformatics.The tissue-specific expression of MCCase was analyzed by quantitation RT-PCR.The results showed MCCase sequence had length of 1 269 bp and coded a protein consisting of 423 amine acids，which was an acid，hydrophilic，unstable protein and was located to mitochondria or cytoplasm.Cassia MCCase had the highest homology with Arabidopsis MCCase.There was a conserved functional domain in MCCase，which was similar to that of acetyl CoA carboxylase.Four highly conserved motifs were found in the conserved domain and might play important role in catalysis.MCCase expressed diferently in various tissues.It showed high expression level in root/hypocotyl and low expression level in other tissues.This might be related to the expressional inhibition of MCCase caused by carbohydrate and light.

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Last Update: 2015-03-03