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Pathogenic Factors Produced by Valsa mali var.mali and Their Relationship With Pathogenicity of Different Strains(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年13期
Page:
118-122
Research Field:
Publishing date:

Info

Title:
Pathogenic Factors Produced by Valsa mali var.mali and Their Relationship With Pathogenicity of Different Strains
Author(s):
LI Chao; LI Bao-hua; LI Gui-fang; WANG Cai-xia
College of Agronomy and Plant Protection,Key Lab of Integrated Crop Pest Management of Shandong Province,Qingdao Agricultural University，Qingdao,Shandong 266109
Keywords:
Valsa mali var.mali; toxins; cell wall degrading enzymes; pathogenicity
PACS:
Q 939.9
DOI:
-
Abstract:
Taking Valsa mali var.mali of strain LXS080601 with high virulence and strain LXS081501 with weak virulence as materials，the enzyme activities and toxins were analyzed using dinitrosalicylic acid (DNS) and high performance liquid chromatography (HPLC) methods,to explore the difference of cell wall degrading enzymes and toxins produced by different strains of Valsa mali and the relationship between pathogenic factors and pathogenicity.The results showed that 5 kinds of toxins were all detected in ‘Fuji’ bark medium and detached branches inoculated with strain LXS080601 including protocatechuic acid，p-hydroxybenzoic acid，p-hydroxybenzene propanoic acid，p-hydroxyacetophenone and phloroglucinol，however，only 3~4 kinds of toxins were produced by strain LXS081501 in two treatments，respectively.The amount of the toxins produced by LXS081501 was significantly lower than that produced by LXS080601，except for phloroglucinol produced in ‘Fuji’ bark medium.In addition，two strains could produce 5 kinds of cell wall degrading enzymes including celluase，β-glucosidase，polymethylgalacturonase，xylanase and polygalacturonase，but the enzyme activities were significantly difference.The enzyme activities produced by LXS080601 were higher than that produced by LXS081501，and the corresponding days were shorter when maximum enzyme activities were detected.The results in this research indicated that a positive relationship existed between enzyme activities，amount and kinds of toxins produced by V.mali and pathogenicity of different strains.

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Last Update: 2014-08-02