Study on the Purification Process of Total Flavonoids of Sedum aizoon L. by Macroporous Adsorption Resin(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Study on the Purification Process of Total Flavonoids of Sedum aizoon L. by Macroporous Adsorption Resin

Author(s):: YANG Yan-jun; WANG Ya-hong; WANG Jun-long; Chemical and Pharmaceutical Engineering Institute,Jilin Institute of Chemical Technology,Jilin,Jilin,132022

Keywords:: Sedum aizoon L.; total flavonoids; purification; macroporous adsorption resin; DM-301

Abstract:: Taking Sedum aizoon L.as material,the dynamic adsorption and desorption performances of five kinds of macroporous adsorption resin including AB-8,HPD-100,D-101,DM-301,DM-130 were researched to choose resin type；With the concentration of total flavonoids as indexes,adsorption and desorption conditions were investigated by single factor experiments.The technological conditions of the purification process of total flavonoids from Sedum aizoon L.by macroporous adsorption resin was investigated.The results showed that DM-301 resin was selected to optimum purification technology,the conditions were：sample solution pH value was 3.0,the concentration was 1.63 mg/mL,the adsorption rate was 2 BV/h，2 BV/h desorption rate using was 70% ethanol solution,desorption solution was 5 BV.The total flavonoids of dry extract was purified from 8.99% to 25.6%,the enrichment ratio was about 3 times.DM-301 macroporous resin used for enriching the total flavonoids of Sedum aizoon L.was better,the optimized operation was simple,the method was reliable.

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Last Update: 2014-08-13