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Optimization Conditions of Tissue Culture of Crocus sativus L.Using Orthogonal Experiment(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年06期
Page:
111-114
Research Field:
Publishing date:

Info

Title:
Optimization Conditions of Tissue Culture of Crocus sativus L.Using Orthogonal Experiment
Author(s):
PENG Hai-junHU Run-huai
College of Forestry and Biological Technology,Zhejiang Agricultural and Forestry University,Lin’an,Zhejiang 311300
Keywords:
Crocus sativus L.orthogonal experimentinduction ratetissue culturerapid propagation
PACS:
R 282.71
DOI:
-
Abstract:
Using corm of Crocus sativus L. as explant,culture medium for the induction of callus,adventitious bud proliferation and the induction of corm were optimized by orthogonal design,through the method of factor index analysis,variance analysis and multiple comparison.The results showed that 1/2MS+NAA 2.0 mg/L+6-BA 0.4 mg/L was the best medium for induction of callus,the appropriate medium for adventitious buds proliferation was 1/2MS medium with NAA 0.6 mg/L and 6-BA 2.0 mg/L,1/2MS+0.5 mg/L NAA+10% banana juice was suitable for corm induction of saffron.

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Last Update: 2014-08-12