Using root tip of diploid and tetraploid Lonicera japonica Thunb M1 as test matericals,karyotypic analysis of diploid and tetraploid Lonicera japonica Thunb were studied.The results showed that diploid Lonicera japonica Thunb:K=2n=2x=18=8m+8sm+2st;tetraploid Lonicera japonica Thunb:K=2n=4x=36=16m+16sm+4st.The chromosome number of the mutant was doubled and proved to be polyploidy.