|Table of Contents|

Effect of Antibiotics on Adventitious Shoot Regeneration of Saintpaulia ionantha (PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年03期
Page:
80-82
Research Field:
Publishing date:

Info

Title:
Effect of Antibiotics on Adventitious Shoot Regeneration of Saintpaulia ionantha
Author(s):
LIU Xiao-dongWANG Ting-tingLIU Qun-lu CHEN Jia-xi
Landscape Architecture College,Northeast Forestry University,Harbin,Heilongjiang 150040
Keywords:
antibioticsSaintpaulia ionanthaadventitious shoot
PACS:
S 681.2
DOI:
-
Abstract:
Taking in vitro leaves of Saintpaulia ionantha as material,the effect of three antibacterial antibiotics (cefotaxime,cephalexin monohydrate,carbenicillin) and three selective antibiotics (kanamycin sulfate,neomycin sulphate,G418) on adventitious shoot regeneration were studied.The results showed that carbenicillin and cefotaxime sodium were better choices to use as antibacterial antibiotic for Saintpaulia ionantha in the process of genetic transformation,and the optimum concentrations were both 300 mg/L.However,cephalexin monohydrate was not a suitable antibiotics because of its toxic effects on the in vitro leaves.Among the three kinds of selective antibiotics,kanamycin sulfate and G418 was more suitable because they had strong inhibition on the shoot regeneration of Saintpaulia ionantha,and the optimum concentrations were 50 mg/L and 5 mg/L,while neomycin sulphate was not a proper antibiotics.

References:

[1] 张卫东 . 室内花卉皇后 - 非洲紫罗兰 [J]. 园林 ,2002(2):20-21.

[2]Kushikawa SHoshino YMii M.Agrobacterium mediated transformation of Saintpaulia ionanthaWendl.[J].Plant Science,2001,161(5):953-960.

[3]傅荣昭,马江生.观赏植物色香形基因工程研究进展-文献综述[J].园艺学报,1995,22(4):381.

[4]何小玲,王金发.观赏花卉的品质基因及其基因工程问题[J].植物生理学通讯,1998,34(6):462-466.

[5]张石宝,胡虹,李树云.花卉基因工程研究进展I:花色[J].云南植物研究,2001,23(4):479-487.

[6]赵云鹏,陈发棣,郭维明.观赏植物花色基因工程研究进展[J].植物学通报,2003,20(1):51-58.

[7]傅荣昭,刘敏.通过根癌农杆菌介导法获得菊花转基因植株[J].植物生理学报,199824(1)72-76.

[8]赵万苓,姜世平,付新生,.利用农杆菌介导法将查尔酮合酶基因导入大岩桐[J].分子植物育种,2006,4(1):45-48.

[9]蔡文燕.根癌农杆菌介导人乳铁蛋白基因转化中国水仙的初步研究[D].福州:福建师范大学,2005.

[10]郑进,康薇,洪华珠.抗生素在农杆菌介导植物转基因中的应用[J].林业科技开发,2006,20(3):8-11.

[11]李杰,刘颖,黄洁虹,.G418和氯霉素作为转基因盐藻的抗生素筛选标记[J].生物技术,2003,13(3):22-23.

[12]王成,王义军,李慧玉,.抗生素对根癌农杆菌的抑菌作用及对新西伯利亚黑杨组培叶片再生的影响[J].东北林业大学学报,2009,37(9):4-7.

[13]吕永杰,李仕贵,周晓禾.观赏兰科植物组培快繁及遗传转化的研究进展[J].中国生物工程杂志,2003,23(10):42-46.

[14]罗素兰,陈若,长孙东亭.番茄组培苗的不同阶段对抗生素和PPT的抗性筛选试验[J].海南大学学报(自然科学版),2003(1):13.

[15]于志水,苘胜军,赵继梅,.杨树转化受体系统再生初步研究及卡那霉素敏感性测定[J].辽宁林业科技,2003(5):9-10.

[16]罗素兰,林皎月,长孙东亭.甘蔗组织培养中不同阶段的抗生素及 PPT 抗性筛选试验[J].海南大学学报(自然科学版),2003(3):15.

[17]张明洲,崔海瑞,舒庆尧,.抗生素对高粱离体培养反应的影响[J].核农学报,2004,18(2):113-117.

[18]张福丽,王贤,李季平,.抗生素对根癌农杆菌抑制作用及对大豆子叶节再生的影响分析[J].广东农业科学,2011(14):76-78.

[19]周小梅,李君剑,赵军良,.抗生素对农杆菌的抑制和对油菜外植体分化的影响[J].西北植物学报,2005,25(1):52-56.

[20]王丽,张俊莲,王蒂,.抗生素对根癌农杆菌的抑菌效果及对烟草叶片分化的影响[J].中国烟草学报,2006,12(1):32-37.

[21]詹立平,姜静,赵鑫,.农杆菌抑菌剂的抑菌效果及其对小黑杨叶片不定芽产生率的影响[J].植物生理学通讯,2004,40(6):689-692.


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Last Update: 2014-08-22