Culture Characteristic Comparison and Condition Control of Fermentation of Two Polysaccharides Agrocybe chaxingu(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Culture Characteristic Comparison and Condition Control of Fermentation of Two Polysaccharides Agrocybe chaxingu

Author(s):: ZHANG Xiao-mei; ZHU Wei-hong; JIA Chunfeng; Department of Biochemistry，Baoding University，Baoding,Hebei 071000

Keywords:: Agrocybe chaxingu; submerged fermentation; polysaccharide; culture characteristic; compare

Abstract:: Taking two samples of polysaccharides Agrocybe chaxingu as material,the culture characteristic and control condition of fermentation were compared.The results showed that a suitable condition of 26℃，pH 6.5，150-250 r/min stir，0.03-0.05 MPa pressure，0.2-0.4 m³/h airflow，and a duration of 96 hours to produce 937-1 080 mg/g of polysaccharide.Tank batch culture shared the same peak period of polysaccharide as that of bottle shaking，but yielded slightly higher.Compared against solid cultivation of fruiting bodies，they yielded 1.3-1.5 times more polysaccharide，and the duration has been shortened by 12-fold.By comparison，the U₂₇ had more chlamydospore，and was more suitable for liquid culture was discovered.By bottle shaking，the fungus balls were small and neat，with diameters between 1.0 and 2.0 mm，density between (1.5-2.5)×103/mL.The submerged fermentation in liquid yielded up to 1 080 mg/g polysaccharide，which was 50% more than that of fruiting，and was proved to be a healthy choice of polysaccharide submerged fermentation.

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Last Update: 2014-12-24