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Study on Extraction Technology of Zucchini Seed Protein Using Double Enzyme Hydrolysis(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年02期
Page:
137-140
Research Field:
Publishing date:

Info

Title:
Study on Extraction Technology of Zucchini Seed Protein Using Double Enzyme Hydrolysis
Author(s):
XIA Guang-hui1; 2; LIU Huan1; HE Wen-bing1
1.Research Center of Changbai Mountain Food Engineering，Tonghua Normal University,Tonghua,Jilin 134002；
2.College of Pharmaceutical and Food Science,Tonghua Normal University，Tonghua,Jilin 134002
Keywords:
zucchini seed; protein; enzyme; extraction; technology
PACS:
S 642. 6
DOI:
-
Abstract:
Taking the zucchini seed as material，using ultrasonic to preprepared,the cellulase and alcalase as multi-enzyme with a mixture of cellulose and alcalase at 2∶1 activity ratio was chosed to study the optimal hydrolysis conditions for double enzyme hydrolysis extraction technology of zucchini seed protein.The results showed that the optimum extraction technology were diluted the substrate with water at the ratio of 1∶20 g/mL，added the multi-enzyme with 7% total dose,hydrolyzed the protein at the pH 7. 5 and temperature of 55℃ for 120 min.The protein extraction efficiency of 74. 2% could be observed under these conditions.

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Last Update: 2014-08-10