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Study on Genetic Specificity of 15 Lentinula edodes Strains(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年18期
Page:
163-166
Research Field:
Publishing date:

Info

Title:
Study on Genetic Specificity of 15 Lentinula edodes Strains
Author(s):
SONG Ying; LIU Na; XIAO Qian-ming; LI Hong
Vegetable Research Institute,Liaoning Academy of Agricultural Sciences,Shenyang,Liaoning 110161
Keywords:
Lentinula edodes; antagonism test; esterase isozyme; cluster analysis
PACS:
S 646
DOI:
-
Abstract:
Genetic specificity of 15 Lentinula edodes strains were studied using antagonism experiment and the technology of esterase isozyme electrophoresis in this paper.The results showed that the quantity of bands of 15 Lentinula edodes strains were in the 8~11 range，enzyme spectrum stability，high repeatability，which showed that esterase isozyme technique could be applied to the taxonomy study of Lentinula edodes species;cluster analysis results showed that in the similar level of 0.41，15 strains aggregated into six types:category 1 includes ‘Hexiang 1’，‘808’ from Lishui city of Zhejiang province and ‘808’ from Dongsheng of Xinbin country among which the strains similarity coefficient of ‘808’ from different place was 100%，testifying the same genetic background and with the same name;category 2 includes ‘Zaofeng 8’ from Dandong city;category 3 includes ‘Xiangyang 2’ and ‘Liaofu 4’;category 4 includes ‘937’ from Liaoning academy of agricultural sciences，‘937’ from Dongsheng of Xinbin country，‘Fuxiang 3’，‘Jinxiang’ and ‘Yongxiang 1’，among which the strains similarity coefficient of ‘937’ from different place was 100%，testifying the same genetic background and with the same name.category 5 includes ‘Pingquan 18’，‘Zhaofeng 8’ from Erdao of Xinbin country and ‘808-2’ from Yongling of Xinbin country;category 6 includes ‘Tiancheng 1’.Cluster analysis results and the results of antagonism test were basically the same.

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Last Update: 2014-11-27