Tissue Culture and Rapid Propagation of Chrysanthemum morifolium(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Author(s):: LI Yun-ling¹; SUN Hu¹; MIAO Jin-shan¹; LIU Jie¹; SUN Ming-mao¹; SUN Hai-liang²; 1.Institute of Horticultural Science and Technology,Weifang University of Science and Technology,Shouguang，Shandong 262700;
2.Shandong Heyi Bio-technology Corporation，Shouguang,Shandong 262700

Keywords:: Chrysanthemum morifolium; stem tip; tissue culture; rapid propagation

Abstract:: With the stem tips of Chrysanthemum morifoliumas the explants,the effects of different disinfection methods and culture mediums on stem tip tissue culture were studied.The results showed that the contamination rate and browning rate of the explants were the lowest by 0.1% mercuric chloride for 8 min.The optimum culture medium for inducing adventitious shoots was MS+6-BA 1.0 mg/L +NAA 0.05 mg/L,the bud induction rate was up to 100%,and the multiple shoots grow rapidly；the optimum rooting culture medium was MS+NAA 0.5 mg/L,it was easier for acclimatizing and transplanting with the sturdy root system.

[1] 薛建平,张爱民,赵丰兰.安徽药菊茎尖组织培养技术的研究[J].中国中药杂志,2002,27(5)：350-353.

[2] 蔡祝南,杨莉莉,彭超美,等.切花菊病毒脱毒及脱毒苗的检测[J].植物病理学报,1992,22(1):34.

[3] 宋瑞林,吴如健,黄兆才.福建省菊花病毒病的初步调查与检测[J].福建农业科技,1997(5):17.

[4] 张伟.切花菊组织培养及快速繁殖的初代研究[J].现代园艺,2012(17):4-5.

[5] 刘庆昌,吴国良.植物细胞组织培养[M].北京.中国农业大学出版社,2003:78-79.

[6] 刘丽辉,王亦民,方顺生.黄山贡菊的组织培养与快速繁殖[J].植物生理学通讯,2001,37(4):314-315.

[7] 刘丽辉.黄山贡菊的脱毒快繁与栽培技术[J].特产研究,2002(1):48-49.

[8] 薛建平,张爱民,常玮.安徽药菊叶片直接再生植株技术的研究[J].中国中药杂志,2004,29(2):132-134.

[9] 薛建平,常玮,张爱民,等.安徽药菊花瓣组织培养技术的研究[J].中国中药杂志,2004,29(3)：211-214.

[10]代丽萍.药菊组织培养研究进展[J].安徽农学通报,2008,14(7):86-88.

Last Update: 2014-08-08