|Table of Contents|

Application of Potassium Pyroantimonate Precipitation on Calcium Distribution in the Style of Lily(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年01期
Page:
78-80
Research Field:
Publishing date:

Info

Title:
Application of Potassium Pyroantimonate Precipitation on Calcium Distribution in the Style of Lily
Author(s):
YAN Jing-yan; TANG Dao-cheng; JU Xiu-ting; ZHANG Yong-yu; MA Qiao-ling
Plateau Flower Research Center,Qinghai University,Xining,Qinghai 810016
Keywords:
lily; calcium distribution; potassium pyroantimonate precipitation
PACS:
Q 949.71+8
DOI:
-
Abstract:
Using lily as material,the conditions and the effects of potassium pyroantimonate precipitation technique to be used to localize calcium in the style of lily,as well as the influences of fixative solutions with different pH were studied.The results showed that calcium precipitates were so obvious in sample sections dealed with in fixative solutions containing potassium pyroantimonate and could be recognized easily under TEM,while no such calcium precipitate particles could be found in the treatment without potassium pyroantimonate.And the cell structure clarity of samples fixed in solutions with pH differing from 7.0 to 7.6 was relatively better than that fixed in solutions with pH 6.8,although no significant differences were found among this range.

References:

[1] Bush D S.Calcium regulation in plant cells and its role in signaling[J].Annu Rev Plant Physiol Plant Mol Biol,1995,46:95-122.

[2] Sanders D,Brownlee C,Harper J F.Communicating with calcium[J].Plant Cell,1999,11：691-706.

[3] 吴志华,郑伟文,庄伟建.钙、钙调蛋白与植物的有性生殖[J].福建农业学报,2002,17（4）:244-247.

[4] 田惠桥,远彤.钙在被子植物受精过程中的作用[J].植物生理学报,2000(26):369-380.

[5] Polito V S.Membrane-associated calcium during pollen grain germination：a microfluorome-tric analysis[J].Protoplasma,1983,117：226-232.

[6] Reiss H D,Herth W.Visualization of Ca2+ gradient in growing pollen tubes of Lilium longiflorumwith chlorotetracycline fluorescence[J].Protoplasma,1978,97:373-377.

[7] Reiss H D,Herth W,Nobiling R.Development of membrane-and calcium-gradients during pollen germination of Lilium longiflorum[J].Planta,1985,163:84-90.

[8] Yu F L,Zhao J,Liang S P,et al.Ultracytochemical localization of calcium in gynoecium and embryo sac of rice[J].Acta Bot Sin,1999,41:125-129.

[9] Zhao J,Yu FL,Liang S P,et al.Changes of calcium distribution in egg cells,zygotes and two-celled proembryos of rice (Oryza sativaL.)[J].Sex Plant Reprod,2002,14:331-337.

[10]谢潮添,杨延红,邱义兰,等.白菜核雄性不育两用系可育和不育花药中Ca2+的分布[J].植物生理与分子生物学学报,2005,31(6):615-624.

[11]尚忠林,马力耕,王学臣,等.细胞外钙调素对百合花粉细胞内钙离子浓度的影响[J].植物学报,2001,43（1）:12-17.

[12]张丰德,王秀玲.现代生物学技术[M].天津:南开大学出版社,2001:52-76.

[13]杨淑娟,宋玉霞,田惠桥,等.枸杞花药发育过程中钙的分布[J].分子细胞生物学报,2006,39(6):516-526.

[14]邓芳,史银连,姚家玲.光敏核不育水稻花药中Ca2+分布及其与花粉育性的相关性研究[J].中国农业科学,2009,42(3):809-815.

[15]彭抒昂.焦锑酸钾沉淀法定位Ca2+在梨生理研究中的应用[J].果树学报,2001,18(3):140-144.

[16]张彦妮,钱灿.12种百合属植物花粉形态扫描电镜观察[J].草业学报,2011,20(5):111-118.

[17]刘选明,何立珍,周朴华,等.百合鳞叶细胞形态发生中胚性细胞的电镜观察[J].湖南农业大学学报,1997,23（1）:30-35.

[18]Wick S M,Hepler P K.Selective localization of intracellular Ca2+ with potassium antimonite[J].J Histochem Cytochem,1982,30:1190-1204.

[19]郭琼杰,孙淑娟.细胞内游离钙离子的测定方法[J].食品与药品,2006,8（12）:25-27.

[20]张国增,李瑞玲,王强.荧光染料在植物细胞Ca2+浓度测定中的应用[J].中国农学通报,2010,26(9):198-201.


Memo

Memo:
-
Last Update: 2014-08-08