|Table of Contents|

Research on the Tissue Culture Technology of Brassica campestris L.ssp. pekinensis Immature Embryos(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年08期
Page:
48-50
Research Field:
Publishing date:

Info

Title:
Research on the Tissue Culture Technology of Brassica campestris L.ssp. pekinensis Immature Embryos
Author(s):
ZHANG Guang-nan; SHAO Deng-kui; HOU Quan-gang; LI Jiang; LI Quan-hui
Qinghai Province Key Laboratory of Vegetable Genetics and Physiology,Institute of Horticulture，Qinghai Academy of Agricultural and Forestry Research,Xining，Qinghai 810016
Keywords:
Brassica campestris L.ssp. pekinensis; immature embryo; tissue culture
PACS:
S 635.103.6
DOI:
-
Abstract:
Taking Brassica campestris L.ssp. pekinensis as material，from the immature embryos’ age，the plant growth regulators level and the root culture medium，the immature embryo culture technology of Brassica campestris L.ssp were studied.The results showed that the age of immature embryos for its tissue culture was 18～25 d,the best plant growth regulators level was MS+NAA 0.6 mg/L+6-BA 1.5 mg/L+30 g/L sucrose+0.7% agar，the best root culture medium was 1/2MS+NAA 0.3 mg/L+30 g/L sucrose+0.7% agar.

References:

[1]Lim H T，You Y S，Park E J，et al.High plant regeneration，genetic stability of regeneration,and genetic transformation of herbicide resistance gene (bar) in Chinese cabbage(Brassica campestris ssp.pekinensis)[J].Acta Hort,1998,459:199-208.

[2]吴沿友,蒋九余,帅世文,等.十字花科几种植物的胚培养研究[J].广西植物,1996(4):367-369,398.

[3]满红,张成合,柳霖坡,等.结球甘蓝二、四倍体间杂交三倍体的获得及细胞学鉴定[J].植物遗传资源学报,2005(4):43-46.

[4]杨慧莹,王鸣刚,武丽娜,等.结球白菜带柄子叶外植体不定芽离体再生能力的遗传分析[J].基因组学与应用生物学,2010(2):332-338.

[5]樊明琴,朱月林,朱丽华.爱知结球白菜组培再生株与实生株的性状比较[J].植物资源与环境学报,2005(1):31-34.

[6]樊明琴,朱月林,朱丽华.结球白菜高效离体子叶不定芽再生的研究[J].南京农业大学学报,2005(1):21-24.


Memo

Memo:
-
Last Update: 2014-08-25