Study on Crypreservation Methods of Lilium spp Pollens(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Author(s):: SHI Ting-ting¹; ZHANG Gui-ling¹; WEN Si-min²; LU Jia-li¹; 1.College of Life Science，Linyi University，Linyi，Shandong 276005；
2.Experimental Management Center，Linyi University，Linyi，Shandong 276005

Abstract:: Taking ‘Sorbonne’ and ‘Siberia’ lily as test materials，the effect of different drying time and different pretreatment temperature and different thawing methods on pollen germination rate after cryopreservation were studied.The results showed that drying time and pretreatment temperature affected the pollen germination after cryopeservation.The germination viability of pollen for 2 h drying pretreatment(with water content of 7.67% and 7.27% respectively) were higher than that of 1，3，4 h.The germination viability of pollen for -20℃ temperature pretreatment was higher than that of CK，0℃,-70℃.Thawing method for 40℃ was better than other two treatments.

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Last Update: 2014-08-23