Effects of Different Hormone Combinations on the Regeneration of ?Four Different Chrysanthemum Genotypes(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Effects of Different Hormone Combinations on the Regeneration of ?Four Different Chrysanthemum Genotypes

Author(s):: GOU Chang; WU Hui; JIANG Da; NING Si-qi; LV Tong; ZHOU Xiao-fu; Key Laboratory of Plant Resources Science and Green Production of Jilin Province，Jilin Normal University，Siping，Jilin 136000

Keywords:: genotypes; chrysanthemum; hormone combinations; regeneration system

Abstract:: Taking four genotypes of chrysanthemum ‘Dajinju’，‘Taohuaxianzi’，‘Ziju’，‘Huangbanqiu’ as materials，petals as explants，the effects of different hormone combinations on cluster buds and roots induction of four chrysanthemum genotypes were investigated.The optimal medium for the regeneration of different chrysanthemum genotypes were screened.The results showed that the best method for sterilization was 4%NaClO 45 s;the best medium for buds induction of ‘Taohuaxianzi’ was MS+6BA 2.0 mg/L+NAA 0.1 mg/L,for ‘Ziju’,it was MS+6BA 1.0 mg/L+NAA 0.1 mg/L,MS+6BA 1.5 mg/L was the best medium for ‘Dajinju’ and ‘Huangbanqiu’；the best medium for rooting of ‘Dajinju’ was 1/2MS+NAA 0.05 mg/L+AC 0.4 g/L,for the others,the best medium was NAA 0.1 mg/L+AC 0.4 g/L.

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Last Update: 2014-08-08