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Tissue Culture and Plant Regeneration of Vaccinium ashei?‘Britewell’(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年19期
Page:
105-107
Research Field:
Publishing date:

Info

Title:
Tissue Culture and Plant Regeneration of Vaccinium ashei?‘Britewell’
Author(s):
ZHU Hong-fen; SHEN Lan; HUANG Jian; ZHANG Guo-fang; WANG Fang; LIU Jian
Institute of Biotechnology Research，Ningbo Academy of Agricultural Sciences，Ningbo，Zhejiang 315040
Keywords:
Vaccinium ashei; tissue culture; plant regeneration
PACS:
S 663.9
DOI:
-
Abstract:
Taking semi-lignified stem with axillary bud of Vaccinium ashei ‘Britewell’ as explants，the effects of the different medias and hormones on plant regeneration were studied.The results showed that WPM was a suitable basic medium.The optimal primary medium for stem with axillary bud was WPM+(0.5～1.0) mg/L ZT，and the induction rate was more than 86%；the suitable proliferation medium was WPM+0.1 mg/L TDZ+0.1 mg/L IBA，the proliferation rate up to 5.48 times.Following the subculture should be at a lower concentration of TDZ (0.02 mg/L and 0.05 mg/L) alternating as well；the rooting medium was 1/2WPM+1.0 mg/L BA，and the effective rooting rate reached 63.2％.

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Last Update: 2014-09-09