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Screen of Sterilized Methods and Inoculation Culture Medium Filter for Explants of Grimmia pilifera(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年10期
Page:
138-140
Research Field:
Publishing date:

Info

Title:
Screen of Sterilized Methods and Inoculation Culture Medium Filter for Explants of Grimmia pilifera
Author(s):
CUI WeiZHANG Mei-juanSHA Wei
College of Life Sciences and Agriculture and Forestry,Qiqihar University,Qiqihar,Heilongjiang 161006
Keywords:
Grimmia piliferatissue culturesterilization methodculture medium
PACS:
S 603.6
DOI:
-
Abstract:
Chosen Grimmia pilifera gametophytic as test material,Benek,MS,Knop as basic culture medium,the tissue culture of disinfection method and inoculation medium types were screened,through pre-culture,pretreatment,had the aid of swirl oscillator.The results indicated that explants pre-culture for about fifteen days,swirl oscillating by 0.025% mercuric chloride solution for 105 seconds cound disinfect effectively for explants.Benek culture medium was suitable for Grimmia pilifera to return to green growth.

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Last Update: 2014-09-05