|Table of Contents|

Plant Regeneration and Callus Induction of Three Lines of Peppers via Cotyledon Culture(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年09期
Page:
108-112
Research Field:
Publishing date:

Info

Title:
Plant Regeneration and Callus Induction of Three Lines of Peppers via Cotyledon Culture
Author(s):
SONG Li-na 1; 2; 3; KANG De-xian3; WANG Hong-mei1; WANG Yi-kui 2; 3; LI Wen-jia2; CAO Zhen-mu4
1.College of Agriculture,Guangxi University,Nanning,Guangxi 530004;
2.Institute of Vegetable Research,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007;
3.Key Laboratory of Guangxi Crop Genetic Improvement and Biotechnology,Nanning,Guangxi 530007;4.Institute of Tropical Crops Genetic Resources,Chinese Academy of Tropical Agricultural Sciences,Danzhou,Hainan 571737
Keywords:
plant regeneration; callus induction; concentration of hormone; AgNO3
PACS:
S 641.303.6
DOI:
-
Abstract:
Taking male sterile line,maintainer line and restorer line of peppers cotyledon as explant,the factors effect of 6-BA,IAA concentration and AgNO3on the in vitro regeneration culture of pepper cotyledon.The results showed that three lines of peppers had differences on callus and adventitious bud induction not merely the best medium of callus and adventitious bud induction but also the number of bud.The bud of R-1 easily outnumber A-1 and B-1；AgNO3didn’t had an obvious effect on callus induction，but 3 mg/L AgNO3 could enhance the rate of adventitious bud differentiation;The higher 6-BA/IAA ratio of ten was conductive to the differentiation of the explants of cotyledons than it was four;1/2MS+IAA 0.2 mg/L+NAA 0.1 mg/L was the optimal medium of rooting by regenerated shoots.

References:

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Last Update: 2014-09-02