The Optimum of HPLC Conditions of Four Kinds of Endogenous Hormones in the Roots of Banana(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: The Optimum of HPLC Conditions of Four Kinds of Endogenous Hormones in the Roots of Banana

Author(s):: BU Jia-jia¹; 2; HU Yu-lin²; WEI Chang-bin²; XIE Jiang-hui²; HE Xin-hua¹; 1.College of Agriculture，Guangxi University,Nanning，Guangxi 530004；
2.South Subtropical Crops Research Institute，Chinese Academy of Tropical Agricultural Science，Zhanjiang，Guangdong 524091

Abstract:: The detection methods of four endogenous hormones [gibberellin acid(GA₃)，indole-3-acetic acid(IAA)，abscisic acid(ABA)，zeatin(ZT)]in roots of bananas by HPLC with UV detector were established.The separation was performed on an Agilent ZORBAX SB-C₁₈ column (250 mm×4.6 mm，5 μm).The optimum chromatographic conditions were as follows：methanol∶acetonitrile∶phosphate buffer solution(pH 3.5)=15∶20∶65 as the mobile phase at 1 mL/min with an injection of 20 μL for GA₃，IAA，ABA at 210 nm；methanol∶acetonitrile∶phosphate buffer solution(pH 3.5)=15∶15∶70 as the mobile phase at 1 mL/min with an injection of 20 μL of ZT at 265 nm.A column temperature of 35℃ was optimum for the separation.The results showed that the recoveries were all high，and this method was rapid and perfect.

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Last Update: 2014-08-30