Establishment of Plantlet Regeneration System of Cotyledon Node for Astragalus membranaceus(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Establishment of Plantlet Regeneration System of Cotyledon Node for Astragalus membranaceus

Author(s):: WANG Shu-bin; QUAN Xue-li; ZHOU Yu-hong; WU Song-quan; College of Agriculture,Yanbian University,Yanji，Jilin 133002

Abstract:: The cotyledon node obtained from aseptic seedling of Astragalus membranaceus were used as the explant and a simple，stable and high efficient regeneration system for A.membranaceus were established.The results showed that the optimum medium for adventitious shoot regeneration was MS+6-BA 1.0 mg/L+NAA 0.10 mg/L，the average number of differential buds per cotyledon node was above 3，and the regenerated frequency was 75.5%.The suitable rooting medium was 1/2MS+IBA 0.5 mg/L and the survive rate was above 75% after transplantation.

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Last Update: 2014-08-30