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In vitro Culture of Superior Individual of Vaccinium uliginosum from Changbai Mountain(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2012年04期
Page:
113-116
Research Field:
Publishing date:

Info

Title:
In vitro Culture of Superior Individual of Vaccinium uliginosum from Changbai Mountain
Author(s):
ZONG Chang-lingZONG Cheng-wenZHAO Wei-weiJIN Bing-kuiCAO Hou-nan
College of Agriculture,Yanbian University,Yanji,Jilin 133002
Keywords:
Vaccinium uliginosumsuperior individualin vitro culturerapid propagation
PACS:
S 666.9
DOI:
-
Abstract:
Taking stem or shoot tip of Vaccinium uliginosum as the explants,in vitro culture of superior individual of Vaccinium uliginosum distributing in Changbai Mountain were studied.The results indicated that the media for inducing shoot clusters was WPM+ZT 2.0 mg/L;the medium for subculture multiplication culture was modified MS+ZT 0.5 mg/L+IBA 0.2 mg/L,and the coefficient of multiplication was 5.3 after culture for 30 days;the medium for the rooting was 1/4 MS+IBA 0.5 mg/L with rooting rate of over 80% after culture for 50 days.Practices transplanting seedling was to tissue culture in sand 20 days,and then transplanting seedlings to the turfy soil: humus soil 1∶2,and survival rate in nursery was up to 78%.

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Last Update: 2014-08-26