Screening and Identification of Bacillus subtilus Strain Bs-18 and Its Biocontrol Evaluation Against Meloidogyne incognita(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2012年01期

Page:

130-132

Research Field:

Publishing date:

Info

Title:

Screening and Identification of Bacillus subtilus Strain Bs-18 and Its Biocontrol Evaluation Against Meloidogyne incognita

Author(s):

ZHANG Yu-qin

Tangshan Vocational and Technical College,Tangshan,Hebei 063000

Keywords:

Bacillus subtilus; Meloidogyne incognita; screening; identification

PACS:

S 432.4+5

DOI:

-

Abstract:

Astrain Bs-18 was got from the soil surrounding plant root,and based on the blast of 16S rDNA sequence, the strain Bs-18 was identified as Bacillus subtilus.In vitro,the root-knot nematodes (Meloidogyne incognita) second stage juveniles (J2) were treated with bacterial suspension of strain Bs-18. Responding to 6 hours treatment, the motility of nematode reached to 99.8% and 95.2% in the bacterial suspension and 10 times dilution of strain Bs-18 respectively. In greenhouse, the quantity of gall and egg mass of the strain Bs-18 treatment were decreased significantly, the galls of 81.8% and 76.5% were less in the treatment of suspension and 10 times dilution of Bs-18 than in the control group. This revealed that the nematocide effectiveness the strain Bs-18 was obviously.

References:

[1]Abad P,Gouzy J,Aury J M,et al.Genome sequence of the metazoan plant-parasitic nematode Meloidogyne incognita[J].Nat Biotechnol,2008,26:909-915.

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