|Table of Contents|

Cloning and Plant Expression Vector Construction of GA20-oxidase Gene from Pear Dwarfing Mutants and Pingguo Pear(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2014年02期
Page:
89-94
Research Field:
Publishing date:

Info

Title:
Cloning and Plant Expression Vector Construction of GA20-oxidase Gene from Pear Dwarfing Mutants and Pingguo Pear
Author(s):
WANG Man; JIN Can; XU Xue; ZONG Cheng-wen; CAO Hou-nan
College of Agricultural,Yanbian University，Yanji，Jinlin 133000
Keywords:
pear dwarf mutant; GA20-oxidase gene; cloning; sequence analysis; plant expression vect
PACS:
S 661. 2
DOI:
-
Abstract:
Taking pear dwarf mutamt and ‘Pingguo’ pear as test material,by RT-PCR technique,cloning and sequencing of GA20ox gene was studied.The results showed that two GA20-oxidase genes which full-length cDNA consist 1 179 nucleotides and contain complete open reading frame (ORF) which encoding a protein of 392 amino acids residue were isolated from pear dwarfing mutant and ‘Pingguo’ pear.Moreover named that PuGA20ox and PbGA20ox.Cloning and plant expression vector construction of GA20-oxidase gene from pear dwarfing mutants and ‘Pingguo’ pear basis for studying the genetic transformation and function of GA20-oxidase gene.

References:

[1]Carrera E,Bou J,Garcia-Martinez J L，et al．Changes in GA 20-oxidase gene expression strongly affect stem length,tuber induction and tuber yield of potato plants[J].Plant,2000(22)：247-256.

[2]张超，孙君灵，贾银华,等.外源激素对一个新的棉花极端矮化突变体AS98植株生长和酶活性的影响[J].中国农业科学,2010(7)：1370-1378.

[3]Lange T.Molecular biology of gibberellin synthesis[J].Planta,1998,204:409-419.

[4]Huang S,Raman A S,Ream J E,et al.Overexpression of 20-oxidase confers a gibberellin-overproduction phenotype in Arabidopsis[J].Plant Physiol，1998，118：773-781.

[5]贾敬贤.梨树矮化密植栽培[M].北京：金盾出版社,2005.

[6]朴永虎.延边小香水梨（Pyrus ussuriensis Maxim）偶然实生矮化突变体的评价研究[D].延吉：延边大学，2010.

[7]金灿.生长调节物质对梨极矮化突变体生长的影响及GA20-氧化酶基因克隆的方法[D].延吉:延边大学,2012.

[8]Lange T，Hedden P，Graebe J E.Expression cloning of a gibberellin 20-oxidase，a multifunctional enzyme involved in gibberellin biosynthesis[J].Proc Natl Acad Sci USA,1994,91:8552-8556.

[9]Wolbang C M，Chandler P M，Smith J J，et al.Auxin from the developing inflo rescence is required forthe biosynthesis of active gib-berellins in barley stems[J].Plant Physiol,2004,134，769-776.

[10]Hedden P,William M.Proebsting.Genetic Analysis of Gibberellin Biosynthesis[J].Plant Physiol,1999,119:365-370.

[11]Stephen D.Jackson P E,James,E C.Regulation of Transcript Levels of a Potato Gibberellin20-Oxidase Gene by Light and Phytochrome B[J].Plant Physiol，2000，124:423-430.

[12]Gawronska H,Yang Y Y,Furukawa K,et al.Effects of Low Irradiance Stress on Gibberellin Levels in Pea Seedlings[J].Plant Cell Physiol,1995，85:87-90.

[13]Peng J，Carol P，Richards D，et al.Arabidopsis GAI gene defines a signaling path-way that negatively regulates gibberellin responses[J].Genes Dev，1997(11)：3194-3205.

[14]李伟，陈晓阳，丁霞.高等植物GA20-氧化酶研究进展[J].生命科学，2003,15(1)：42-46.

[15]Tan X,Yang H,Qiao D J,et al.Construction of si RNA plant expression vector interfered with GA 20-oxidase and production of dwarf tobacco[J].Chin J Environ Biol，2008，14(1):48-52.

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Last Update: 2014-08-10