|Table of Contents|

Seed Quality and Germitation Characteristics of Cymbidium erythraeum×C.mastersii Hybrids

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2026年4
Page:
69-75
Research Field:
Publishing date:

Info

Title:
Seed Quality and Germitation Characteristics of Cymbidium erythraeum×C.mastersii Hybrids
Author(s):
ZHOU Xueqing1; CHEN Xueda1; SHAN Xiaodan2; WANG Rui1; ZHAO Guanfei3; XING Zhen1
(1.College of Resources and Environment，Xizang Agricultural and Animal Husbandry University/Xizang Orchidaceae Research Center/Key Laboratory of Forest Ecology in Xizang Plateau (Ministry of Education)，Linzhi，Xizang 860000；2.Institute of Botany，Chinese Academy of Sciences，Beijing 100093；3.Vegetable Research Institute，Xizang Academy of Agricultural and Animal Husbandry Sciences，Lhasa，Xizang 850032)
Keywords:
Cymbidium erythraeum; Cymbidium mastersii; hybridization; aseptic germination; proliferation and differentiation
PACS:
S682.31
DOI:
10.11937/bfyy.20252417
Abstract:
Taking hybrid seeds of Cymbidium erythraeum Lindl.(♀)×Cymbidium mastersii Griff.ex Lindl.(♂) as test material，seed morphology and embryo rate were determined using a stereomicroscope.The study compared sterile germination and early primary bulbil formation on five basic media (MS，1/2MS，1/4MS，KC，and Huabao No.1) to screen for the optimal medium.Using the primary bulbils as explants，three treatment groups were set up,dark cultivation for 24 hours (Group A)，12 hours light+12 hours dark (Group B)，and 12 hours light+6-BA (1.0 mg·L-1)+NAA (0.5 mg·L-1) (Group C).After 120 days of cultivation，the primary bulbil proliferation coefficient and differentiation rate were measured,in order to provide reference for the rapid propagation and breeding of hybrid germplasm resources of Cymbidium erythraeum and Cymbidium mastersii.The results showed that the hybrid of Cymbidium mastersii (♂)×Cymbidium erythraeum (♀) produced significantly larger seeds than the backcross of Cymbidium mastersii (♀)×Cymbidium erythraeum (♂)，though the embryo size was similar，with no significant difference in embryo rate (93.65% and 97.65%，respectively).The KC medium promoted both seed germination and primary bulbil formation for both crosses，with Group C showing the highest proliferation coefficient and differentiation rate.The proliferation coefficients for the two crosses were 4.50±0.44 and 18.00±1.09，respectively，and the differentiation rates were 0.41%±0.05% and 0.80%±0.06%.

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Last Update: 2026-03-12