|Table of Contents|

Untargeted Metabolomics Profiling of Metabolic Variations During Fruiting Body Formation in Pleurotus pulmonarius

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2025年24
Page:
36-45
Research Field:
Publishing date:

Info

Title:
Untargeted Metabolomics Profiling of Metabolic Variations During Fruiting Body Formation in Pleurotus pulmonarius
Author(s):
YAN Jing; WANG Weike; XIN Ya; ZHOU Zufa
(Hangzhou Acdemy of Agricultural Sciences，Hangzhou，Zhejiang 310024)
Keywords:
Pleurotus pulmonarius; fruiting body formation; metabolic characteristics; untargeted metabolomics
PACS:
S 646
DOI:
10.11937/bfyy.20251732
Abstract:
Taking Pleurotus pulmonarius as the test material,an untargeted metabolomics study based on liquid chromatography-mass spectrometry (LC-MS) was conducted to analyze metabolic differences before and after fruiting body formation，aiming to identify key metabolic pathways and biomarkers associated with this process.The results showed that，cultivation bags of P.pulmonarius were subjected to low-temperature stimulation to induce fruiting，and the entire process-from mycelial growth to primordium formation-was divided into four stages (S0，S1，S2，and S3).Mycelia from each stage were analyzed using LC-MS.A total of 18 262 metabolites were identified in both positive and negative ion modes.Significant differences were observed in metabolite profiles,102 and 259 differentially expressed metabolites (DEMs) between S0 and S1 388 and 269 DEMs between S0 and S2,3 528 and 4 029 DEMs between S0 and S3.Further analysis revealed that nucleotide metabolism，amino acid metabolism，amide metabolism，and organic acid metabolism were closely linked to fruiting body formation.Multivariate statistical analysis highlighted substantial changes in metabolic pathways and metabolite levels during the transition from vegetative growth (S0) to fruiting body maturation (S3).These findings provided critical insights into the molecular and metabolic dynamics driving Pleurotus pulmonarius development.

References:

［1］王小谭.碳氮源对秀珍菇液体培养及胞外酶活性的影响［D］.合肥:安徽农业大学,2019.［2］ZHANG Z,WEN J,LI J,et al.The evolution of genomic and epigenomic features in two Pleurotus fungi［J］.Scientific Reports,2018,8(1):8313.［3］闫静,王伟科,袁卫东,等.温度对秀珍菇生长发育及胞外酶活性的影响［J］.浙江大学学报(农业与生命科学版),2020,46(2):161-167.［4］SONG B,YE J,SOSSAH F L,et al.Assessing the effects of different agro-residue as substrates on growth cycle and yield of Grifola frondosa and statistical optimization of substrate components using simplex-lattice design［J］.AMB Express,2018,8(1):46.［5］SEKAN A S,MYRONYCHEVA O S,KARLSSON O,et al.Green potential of Pleurotus spp.in biotechnology［J］.PeerJ,2019,7:e6664.［6］WU N,TIAN F,MOODLEY O,et al.Optimization of agro-residues as substrates for Pleurotus pulmonarius production［J］.AMB Express,2019,9(1):184.［7］MILOVANOVIC I,LAJIN B,BRAEUER S,et al.Simultaneous selenium and sulfur speciation analysis in cultivated Pleurotus pulmonarius mushroom［J］.Food Chemistry,2019,279:231-236.［8］SATO M,MIYAGI A,YONEYAMA S,et al.CE-MS-based metabolomics reveals the metabolic profile of Maitake mushroom (Grifola frondosa) strains with different cultivation characteristics［J］.Bioscience,Biotechnology,and Biochemistry,2017,81(12):2314-2322.［9］DU F,ZOU Y,HU Q,et al.Metabolic profiling of Pleurotus tuoliensis during Mycelium physiological maturation and exploration on a potential indicator of mycelial maturation［J］.Frontiers in Microbiology,2019,9:3274.［10］TANG L,SHANG J,SONG C,et al.Untargeted metabolite profiling of antimicrobial compounds in the brown film of Lentinula edodes Mycelium via LC-MS/MS analysis［J］.ACS Omega,2020,5(13):7567-7575.［11］SCHRIMPE-RUTLEDGE A C,CODREANU S G,SHERROD S D,et al.Untargeted metabolomics strategies-challenges and emerging directions［J］.Journal of the American Society for Mass Spectrometry,2016,27(12):1897-1905.［12］PETERS D L,WANG W,ZHANG X,et al.Metaproteomic and metabolomic approaches for characterizing the gut microbiome［J］.PROTEOMICS,2019,19(16):1800363.［13］ZHANG G,WANG H,XIE W,et al.Comparison of triterpene compounds of four botanical parts from Poria cocos (Schw.) wolf using simultaneous qualitative and quantitative method and metabolomics approach［J］.Food Research International,2019,121:666-677.［14］王伟科,袁卫东,方献平,等.秀珍菇不同生长发育阶段蛋白质组学分析［J］.浙江大学学报(农业与生命科学版),2017,43(5):527-535.［15］刘芹,师子文,吴杰,等.基于非靶代谢组学的糙皮侧耳子实体发育的关联物质及主要代谢通路分析［J］.菌物学报,2024,43(2):56-70.［16］陈辉.氧化还原物质对斑玉蕈子实体形成及其发育影响的机制研究［D］.合肥:合肥工业大学,2019.［17］杨球普,徐明,魏珊,等.工厂化栽培海鲜菇菌包的代谢产物动态变化特征［J］.北方园艺,2024(1):121-129.［18］刘金容,张艳成,刘演,等.灰树花菌丝体不同培养时期代谢组学分析［J］.广西植物,2024,44(7):1337-1351.［19］王伟科,陆娜,闫静,等.秀珍菇不同生长阶段在低温刺激后的转录组及差异基因表达分析［J］.农业生物技术学报,2020,28(10):1837-1848.

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Last Update: 2026-01-09