|Table of Contents|

Cloning and Expression Analysis of the Coding Region of CsSTK in Tea Plants

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2025年21
Page:
1-7
Research Field:
Publishing date:

Info

Title:
Cloning and Expression Analysis of the Coding Region of CsSTK in Tea Plants
Author(s):
LIU Yufei; PANG Dandan; CHEN Chunlin; TIAN Yiping; DENG Shaochun; CHEN Linbo
(Tea Research Institute，Yunnan Academy of Agricultural Sciences/Yunnan Provincial Key Laboratory for Tea Science/Yunnan Technology Engineering Research Center of Tea Germplasm Innovation and Supporting Cultivation，Kuming，Yunnan 650200)
Keywords:
tea plants; CsSTK; gene cloning; expression analysis; flower development
PACS:
S 571.1
DOI:
10.11937/bfyy.20251038
Abstract:
Taking the tea plant cultivar ‘Yuncha 1’ and ‘Yuncha Qirui’ as experimental materials，the coding sequence of the CsSTK was cloned via RT-PCR technique，followed by bioinformatic analysis and expression pattern investigation，in order to provide reference for a deeper understanding of the molecular mechanisms by which CsSTK regulates reproductive development in tea plants.The results showed that the CsSTK gene had an open reading frame of 696 bp，encoding 231 amino acids.The encoded protein contains MADS-box and K-box domains，classifying it within the MADS-box family.Phylogenetic analysis revealed that CsSTK was highly homologous to STK proteins from other plants，with the closest relationship to the CjSTK protein of Camellia japonica.Tissue-specific expression analysis showed that CsSTK was highly expressed in the flowers and fruits of the tea plant，while its expression was significantly reduced in the flowers of the gynoecium-deficient germplasm ‘Yuncha Qirui’，suggested its potential involvement in the development of flowers and fruits，particularly closely related to gynoecium development.Protein interaction analysis indicated that CsSTK may participate in the regulation of flower development through interactions with several MADS-box proteins such as SEP3，AGL80，and BEL1，and was influenced by ubiquitination modifications.

References:

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Last Update: 2025-11-25