|Table of Contents|

Genome-wide Identification，Characterization，and Expression Profiling of the WRKY Gene Family in Mesembryanthemum crystallinum

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2025年20
Page:
31-41
Research Field:
Publishing date:

Info

Title:
Genome-wide Identification，Characterization，and Expression Profiling of the WRKY Gene Family in Mesembryanthemum crystallinum
Author(s):
SUN Shuqi; ZOU Zhiyong; LIU Xiaohan; ZHAO Lina; SONG Xiaoming; LI Nan
(College of Life Sciences，North China University of Science and Technology，Tangshan,Hebei 063210)
Keywords:
Mesembryanthemum crystallinum; WRKY gene family; gene family identification; bioinformatics analysis; expression analysis
PACS:
S 649
DOI:
10.11937/bfyy.20250931
Abstract:
Taking halophyte Mesembryanthemum crystallinum as the test materials，47 McWRKY transcription factor genes were systematically identified through whole-genome data and bioinformatics analysis，in order to provide reference for genetic improvement of crop salt tolerance.The results showed that the gene encoding proteins range from 147 to 1 196 amino acids (aa) with isoelectric points of 4.92-9.88.Phylogenetic analysis classified these genes into three major evolutionary groups (Group Ⅰ-Ⅲ)，with Group Ⅱ further subdivided into five subgroups.Chromosomal mapping revealed their uneven distribution across nine chromosomes，and 42 genes contained intronic structures.Promoter analysis identified 1 527 stress-responsive cis-acting elements (e.g.，ABRE，MYB，and W-box)，while collinearity analysis detected 14 segmental duplication events.Transcriptomic profiling under gradient salt stress (0-500 mmol·L-1 NaCl) demonstrated dose-dependent upregulation of seven McWRKY genes.These findings suggest that structural diversity，cis-element enrichment，and dynamic expression regulation of McWRKY genes contribute to salt tolerance in M.crystallinum，with McWRKY22，McWRKY35 potentially acting as core regulators to activate salt-responsive signaling pathways.

References:

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Last Update: 2025-11-11