|Table of Contents|

Pathogen Identification and In Vitro Inhibiton of Root Rot Disease From Hippeastrum rutilum

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2025年18
Page:
95-101
Research Field:
Publishing date:

Info

Title:
Pathogen Identification and In Vitro Inhibiton of Root Rot Disease From Hippeastrum rutilum
Author(s):
XU Yan; LI Yudie; CHEN Siqi; WANG Jiamei; TIAN Xuejun; YI Qingping
(College of Food and Biology,Jingchu University of Technology/Hubei Engineering Research Center for Specialty Flowers Biological Breeding，Jingmen，Hubei 448000)
Keywords:
Hippeastrum rutilum; root rot disease; isolation; identification; in vitro inhibition
PACS:
S 436.8
DOI:
10.11937/bfyy.20250616
Abstract:
Taking different varieties of Hippeasterum rutilum diseased tissues as the test materials,the pathogenic bacteria were isolated by tissue isolation method,and the isolated pathogenic bacteria were observed morphologically and identified by ITS sequence analysis.The in vitro inhibitory effects of five pesticides (hlorothalonil,carbendazim,mancozed,thiophanate-methyl,and dimetachlone) at three different concentrations (0.01,0.02,0.04 g·mL-1) on the isolated pathogenic bacteria were explored,in order to provide reference for the isolation,identification,and prevention of root rot pathogens in Hippeasterum rutilum.The results showed that a total of 25 single bacterial strains were isolated from the samples of the Hippeastrum rutilum diseased plant，which were classified into three types of bacteria based on their colony characteristics.ITS sequence analysis revealed that the 25 single bacterial strains actually only had three gene sequences，and were preliminarily identified by BLAST as belonging to the genus Fusarium.In addition，the in vitro inhibitory effects of five pesticides on three types strains of root rot disease from Hippeastrum rutilum were different，with seven treatments showing significant inhibitory effects to varying degrees.

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Last Update: 2025-10-14