|Table of Contents|

Identification and Expression Analysis of V-ATPase Family Genes in Melon

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2025年16
Page:
1-9
Research Field:
Publishing date:

Info

Title:
Identification and Expression Analysis of V-ATPase Family Genes in Melon
Author(s):
GAO Ruina1; GAO Chao2; SUN Jianlei2; LIU Peng2; LI Shuai2; HAN Deguo1
(1.College of Horticulture and Landscape Architecture，Northeast Agricultural University，Harbin,Heilongjiang 150030；2.Vegetable Research Institute,Shandong Academy of Agricultural Sciences/Key Laboratory of Yellow and Huaihai Facility Horticulture Engineering,Ministry of Agriculture and Rural Affairs/Key Laboratory Breeding for Major Field Vegetables in Shandong Province,Jinan,Shandong 250100)
Keywords:
melon; V-ATPase; gene family; expression analysis
PACS:
S 634.101
DOI:
10.11937/bfyy.20245005
Abstract:
Taking the melon inbred line ‘B3’ as the test material,the melon V-ATPase (VHA) family genes were identified by bioinformatics methods.The physicochemical properties,chromosomal localization,subcellular localization,collinearity,cis-acting elements of the promoter,and tissue expression were analyzed,in order to provide reference for clarifying the function and mechanism of action of VHA.The results showed that the melon VHA gene family comprised 20 members.These 20 VHA genes were unevenly distributed across 9 chromosomes.Subcellular localization prediction showed that their locations were relatively complex，being distributed in various compartments including the cell membrane，nucleus，vacuole，and chloroplast.Based on phylogenetic tree analysis，the melon VHA gene family could be divided into 12 subfamilies.Analysis of promoter cis-acting elements showed that the members contained response elements for light，auxin，abscisic acid，salicylic acid，gibberellin，methyl jasmonate，low temperature，anaerobic induction，and drought.Collinearity analysis revealed 12 collinear gene pairs between melon and Arabidopsis thaliana，while only 2 collinear gene pairs were found within the melon species.Conservative domain analysis results demonstrated that the number and type of conserved motifs varied among the protein sequences of VHA genes，and the number of introns ranged from 1 to 17.Tissue expression analysis showed that 15 VHA family genes were expressed at varying levels in different tissues，while 5 VHA genes were undetectable in melon.Most gene members exhibited higher expression levels in fruit compared to that in flowers，roots，stems and leaves.Notably，MELO3C014920.2 and MELO3C010992.2 were highly expressed at various developmental stages of fruit.Indicating that,they might play important regulatory roles in fruit development of melon.

References:

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Last Update: 2025-09-19