|Table of Contents|

Establishment of ‘Poinsettia’ Strawberry Regeneration System and Tissue Culture Rapid Propagation System

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2025年7
Page:
9-17
Research Field:
Publishing date:

Info

Title:
Establishment of ‘Poinsettia’ Strawberry Regeneration System and Tissue Culture Rapid Propagation System
Author(s):
MIAO ShuangyuWANG Lijuan
(Department of Horticulture,Tianjin Agricultural College,Tianjin 300392)
Keywords:
strawberryleaf regenerationstem tip rapid propagationhormone
PACS:
S 668.4
DOI:
10.11937/bfyy.20244067
Abstract:
Taking ‘Poinsettia’ strawberry leaves and stem tips as test materials,the effects of exogenous hormones on healing tissue induction,adventitious shoot differentiation,proliferation and rooting culture were studied by using the in vitro culture technique,so as to establish an in vitro regeneration system and a rapid multiplication system for ‘Poinsettia’ strawberry,in order to provide reference for the rapid propagation and genetic transformation of ‘Poinsettia’ strawberry seed resources.The results showed that the combination of 1.5 mg·L-1 TDZ and 0.1 mg·L-1 IBA was the most suitable for callus induction and indeterminate shoot differentiation in the indeterminate shoot induction stage;the most suitable medium for the indeterminate shoot proliferation stage was MS+1.0 mg·L-1 TDZ+0.1 mg·L-1 IBA;in the indeterminate shoot rooting stage,the most suitable medium for the rooting medium was MS+0.1 mg·L-1 IBA.The most suitable medium for the primary culture of stem tip was MS+1.0 mg·L-1 6-BA+0.1 mg·L-1 IBA,with a germination rate of 80%;the optimum proliferation medium for obtaining clumped shoots was MS+0.5 mg·L-1 6-BA+0.1 mg·L-1 IBA,with a proliferation coefficient of 4.20;and the optimum rooting medium was 1/2MS,with a rooting rate as high as 100%.

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Last Update: 2025-04-16