|Table of Contents|

Effects of Bacillus velezensis T4 on the Seed Germination and Seedling Growth Characteristics of Cucumber

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2025年7
Page:
58-65
Research Field:
Publishing date:

Info

Title:
Effects of Bacillus velezensis T4 on the Seed Germination and Seedling Growth Characteristics of Cucumber
Author(s):
SHEN Jun1; GU Qingyi2; SHENG Minghui1; LIU Dengpan1; WANG Jie1; LIU Songhu1
(1.Horticulture College，Xinyang Agriculture and Forestry University，Xinyang，Henan 464000；2.School of Pharmacy，Xinyang Agriculture and Forestry University，Xinyang，Henan 464000)
Keywords:
Bacillus velezensis; cucumber; physiological index
PACS:
S 642.2
DOI:
10.11937/bfyy.20243933
Abstract:
Taking ‘Zhongnong 26’ cucumber seeds as experimental materials,the effects of different concentrations of Bacillus velezensis T4 suspensions (6.88×108,3.44×108,6.88×107,6.88×106,6.88×105 cfu · mL-1) on cucumber seed germination,seedling morphological indicators and physiological characteristics were studied by seed soaking and root irrigation methods,with clear water as the control (CK),in order to evaluate their promotive effects in cucumber cultivation and provide reference for the application of T4 strain in bio-fertilizers.The results showed that compared with CK，6.88×106 cfu·mL-1 and 6.88×105 cfu·mL-1 treatments significantly increased the germination index and germination potential of cucumber seeds.The stem diameter，root volume，leaf area and dry weight of cucumber seedlings were significantly increased by 6.88×105 cfu·mL-1 treatment after 7 days and 14 days of root irrigation.After 21 days of root irrigation，the chlorophyll content of 6.88×106 cfu·mL-1 treatment was significantly higher than that of CK.The content of carotenoids in 6.88×106 cfu·mL-1 treatment was significantly higher than that in CK at 7 days，14 days and 21 days.The soluble sugar content in 6.88×105 cfu·mL-1 treatment was significantly higher than that in CK，and the soluble protein content in 3.44×108 cfu·mL-1 treatment was significantly higher than that in CK.After 14 days of root irrigation，the POD activity of 6.88×106 cfu·mL-1 treatment was significantly higher than that of CK，and the malondialdehyde content (MDA) of different treatments was significantly lower than that of CK.In summary，B.velezensis T4 was found to improve the physiological and biochemical characteristics of cucumber.It enhanced the stress resistance of cucumber seedlings enhanced，and promoted the growth of seedlings.In particular，the growth-promoting effect was more pronounced at a concentration of 6.88×105 cfu·mL-1.

References:

［1］周可,魏冬苓,张峰峰,等.解淀粉芽胞杆菌HN对番茄、黄瓜病原菌的抑制作用［J］.天津师范大学学报(自然科学版),2019,39(4):51-57.［2］邱光,张新凤,李建伟,等.解淀粉芽孢杆菌B1619对草莓保苗促生效果［J］.江苏农业科学,2019,47(4):98-100.［3］张莉,韩玲娟,张毅,等.解淀粉芽孢杆菌QST713对低钙胁迫下黄瓜幼苗生长及光合作用的影响［J］.山西农业科学,2022,50(3):336-345.［4］王英国,王军华,权春善,等.解淀粉芽孢杆菌抗菌活性物质的分离纯化及抑菌活性研究［J］.中国生物工程杂志,2007，27(12):41-45.［5］钱婷,叶建仁.巨大芽孢杆菌ZS-3溶无机磷机制及其对樟树的促生作用［J］.生物技术通报,2020,36(8):45-52.［6］徐桑尔,卢鹏,金莉萍,等.多粘类芽孢杆菌防治铁皮石斛细菌性叶斑病及促生效果［J］.浙江农业科学,2016,57(12):2033-2034.［7］圆圆,张虹,苏道拉呼,等.抑制黄瓜枯萎病的链霉菌S-101及其生防作用研究［J］.中国生物防治学报,2019,35(5):813-820.［8］卢彩鸽,董红平,张殿朋,等.解淀粉芽孢杆菌MH71摇瓶发酵培养基及发酵条件优化［J］.中国生物防治学报,2015,31(3):369-377.［9］牟玉梅,李菲,范高领,等.辣椒种子中拮抗青枯病菌内生细菌的促生功能分析［J］.中国瓜菜,2020,33(7):56-60.［10］张学青,胡蒙爱,王晓卓,等.壳寡糖、芽孢杆菌、香菇多糖复合对黄瓜幼苗生长和低温抗性的影响［J］.江苏农业科学,2023,51(18):156-162.［11］褚睿,李昭轩,张学青,等.黄瓜枯萎病拮抗芽孢杆菌的筛选、鉴定及其生防潜力［J］.生物技术通报,2023,39(8):262-271.［12］宋小颖,董胜旗,于海阔,等.阿氏芽孢杆菌解硅微生物菌剂MB35-5对日光温室黄瓜土壤有效硅含量及产量的影响［J］.河北农业科学,2023,27(4):78-82.［13］陈丹阳,张振洋,黎剑,等.枯草芽孢杆菌发酵玉米黄粉制备可溶性肽［J］.食品与发酵工业,2019,45(14):78-83.［14］尉辉,孟婧,刘亚琦,等.EM菌浸种对低温处理黄瓜萌发和幼苗生长的影响［J］.山东农业工程学院学报,2022,39(9):35-38.［15］张燕,任毛飞,胥梦洋,等.硫酸铜对甜瓜种子萌发及幼苗生长的影响［J］.北方园艺,2022(11):37-43.［16］项倩,吴磊,徐若涵,等.不同温度下染病番茄叶片SPAD和叶绿素含量的相关性［J］.北方园艺,2022(18):8-15.［17］谷清义,王妍佳,李梦楠,等.枯草芽孢杆菌BSCY-1对黄瓜种子萌发和幼苗生长特性的影响［J］.北方园艺,2024(10):17-23.［18］XU Q M,CHENG J S,GE Z Q,et al.Antioxidant responses to oleic acid in two-liquid-phase suspension cultures of Taxus cuspidate［J］.Applied Biochemistry and Biotechnology,2005,125(1):11-26.［19］姚悦,余庭庭,张瑞,等.茶树叶提取物对富士苹果耐贮性的影响［J］.北方园艺,2023(21):93-100.［20］HANDELSMAN J,STABB E V.Biocontrol of soilborne plant pathogens［J］.The Plant Cell,1996,8(10):1855-1869.［21］宗英,赵月菊,刘阳,等.一株贝莱斯芽孢杆菌抑制禾谷镰刀菌的研究［J］.核农学报,2018,32(2):310-317.［22］WANG J J,XU S,YANG R,et al.Bacillus amyloliquefaciens FH-1 significantly affects cucumber seedlings and the rhizosphere bacterial community but not soil［J］.Scientific Reports,2021,11(1):12055.［23］张德珍,李婷婷,代惠洁,等.解淀粉芽孢杆菌JF-1对黄瓜的防病促生作用［J］.北方园艺,2020(10):16-21.［24］吴玉洪,冷超凡,陈莹莹,等.贝莱斯芽孢杆菌BMF03对黄瓜幼苗促生长作用及其生物学功能研究［J］.东北农业科学,2023,48(3):106-111.［25］邸桂俐,高艳玲,张抒,等.外源Ca2+对玉米幼苗镉胁迫的缓解效应［J］.现代化农业,2024(2):27-30.［26］马爽.贝莱斯芽孢杆菌K-9的筛选及其对马铃薯疮痂病生防机制的研究［D］.大庆:黑龙江八一农垦大学,2023.［27］孙小玲,许岳飞,马鲁沂,等.植株叶片的光合色素构成对遮阴的响应［J］.植物生态学报,2010,34(8):989-999.［28］林达定,张国防,于静波,等.芳樟不同无性系叶片光合色素含量及叶绿素荧光参数分析［J］.植物资源与环境学报,2011,20(3):56-61.［29］王东明,贾媛,崔继哲.盐胁迫对植物的影响及植物盐适应性研究进展［J］.中国农学通报,2009,25(4):124-128.

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Last Update: 2025-04-16